qusage

DOI: 10.18129/B9.bioc.qusage    

qusage: Quantitative Set Analysis for Gene Expression

Bioconductor version: Release (3.6)

This package is an implementation the Quantitative Set Analysis for Gene Expression (QuSAGE) method described in (Yaari G. et al, Nucl Acids Res, 2013). This is a novel Gene Set Enrichment-type test, which is designed to provide a faster, more accurate, and easier to understand test for gene expression studies. qusage accounts for inter-gene correlations using the Variance Inflation Factor technique proposed by Wu et al. (Nucleic Acids Res, 2012). In addition, rather than simply evaluating the deviation from a null hypothesis with a single number (a P value), qusage quantifies gene set activity with a complete probability density function (PDF). From this PDF, P values and confidence intervals can be easily extracted. Preserving the PDF also allows for post-hoc analysis (e.g., pair-wise comparisons of gene set activity) while maintaining statistical traceability. Finally, while qusage is compatible with individual gene statistics from existing methods (e.g., LIMMA), a Welch-based method is implemented that is shown to improve specificity. For questions, contact Chris Bolen (cbolen1@gmail.com) or Steven Kleinstein (steven.kleinstein@yale.edu)

Author: Christopher Bolen and Gur Yaari, with contributions from Juilee Thakar, Hailong Meng, Jacob Turner, Derek Blankenship, and Steven Kleinstein

Maintainer: Christopher Bolen <cbolen1 at gmail.com>

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("qusage")

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("qusage")

 

PDF	R Script	Running qusage
PDF		Reference Manual

Details

biocViews	GeneSetEnrichment, Microarray, RNASeq, Software
Version	2.12.0
In Bioconductor since	BioC 2.13 (R-3.0) (4.5 years)
License	GPL (>= 2)
Depends	R (>= 2.10), limma(>= 3.14), methods
Imports	utils, Biobase, nlme, lsmeans
LinkingTo
Suggests
SystemRequirements
Enhances
URL	http://clip.med.yale.edu/qusage
Depends On Me
Imports Me
Suggests Me	SigCheck
Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package	qusage_2.12.0.tar.gz
Windows Binary	qusage_2.12.0.zip
Mac OS X 10.11 (El Capitan)	qusage_2.12.0.tgz
Source Repository	git clone https://git.bioconductor.org/packages/qusage
Package Short Url	http://bioconductor.org/packages/qusage/
Package Downloads Report	Download Stats