Welcome to MiDAS. This tutorial is supposed to help you get started with your analyses of immunogenetic associations. We will work with a simulated data set of 500 patients and 500 controls with a binary disease diagnosis.
We also have high resolution HLA alleles (4 - 6 digit), and presence/absence calls for KIR genes.
First, let’s import the phenotype data and HLA calls using MiDAS import functions. MiDAS will check for correct nomenclature of HLA. We can also define 4-digit resolution for HLA alleles as import format, which means that alleles with higher resolution will be reduced.
dat_pheno <-
read.table(
file = system.file("extdata", "MiDAS_tut_pheno.txt", package = "midasHLA"),
header = TRUE
)
dat_HLA <-
readHlaCalls(
file = system.file("extdata", "MiDAS_tut_HLA.txt", package = "midasHLA"),
resolution = 4
)
Let’s take a look at the imported HLA data tables:
ID | A_1 | A_2 | B_1 | B_2 | C_1 | C_2 | DPA1_1 | DPA1_2 | DPB1_1 | DPB1_2 | DQA1_1 | DQA1_2 | DQB1_1 | DQB1_2 | DRA_1 | DRA_2 | DRB1_1 | DRB1_2 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
C001 | A*25:01 | A*26:01 | B*07:02 | B*18:01 | C*12:03 | C*07:02 | DPA1*01:03 | DPA1*01:03 | DPB1*02:01 | DPB1*04:01 | DQA1*05:05 | DQA1*01:02 | DQB1*06:02 | DQB1*03:01 | DRA*01:02 | DRA*01:02 | DRB1*15:01 | DRB1*12:01 |
C002 | A*02:01 | A*02:324 | B*50:01 | B*18:01 | C*06:02 | C*12:03 | DPA1*01:03 | DPA1*01:03 | DPB1*04:02 | DPB1*04:02 | DQA1*02:01 | DQA1*05:05 | DQB1*02:02 | DQB1*03:01 | DRA*01:01 | DRA*01:01 | DRB1*07:01 | DRB1*11:04 |
C003 | A*24:02 | A*24:04 | B*46:01 | B*40:06 | C*01:03 | C*15:02 | DPA1*02:02 | DPA1*02:01 | DPB1*05:01 | DPB1*14:01 | DQA1*01:04 | DQA1*01:03 | DQB1*06:01 | DQB1*05:02 | DRA*01:02 | DRA*01:01 | DRB1*14:07 | DRB1*08:03 |
C004 | A*01:01 | A*24:02 | B*08:01 | B*15:01 | C*07:01 | C*03:03 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*03:01 | DQA1*01:03 | DQA1*01:02 | DQB1*06:04 | DQB1*06:03 | DRA*01:02 | DRA*01:01 | DRB1*13:01 | DRB1*13:02 |
C005 | A*01:01 | A*25:01 | B*18:01 | B*08:01 | C*12:03 | C*07:01 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*23:01 | DQA1*05:01 | DQA1*01:02 | DQB1*02:01 | DQB1*06:02 | DRA*01:02 | DRA*01:02 | DRB1*03:01 | DRB1*15:01 |
C006 | A*03:01 | A*01:01 | B*07:02 | B*08:01 | C*07:01 | C*07:02 | DPA1*01:03 | DPA1*01:03 | DPB1*57:01 | DPB1*271:01 | DQA1*01:02 | DQA1*01:01 | DQB1*06:04 | DQB1*05:01 | DRA*01:01 | DRA*01:02 | DRB1*13:02 | DRB1*01:02 |
C007 | A*01:01 | A*02:01 | B*15:01 | B*08:01 | C*07:01 | C*03:03 | DPA1*02:01 | DPA1*01:03 | DPB1*04:02 | DPB1*13:01 | DQA1*05:01 | DQA1*03:01 | DQB1*02:01 | DQB1*03:02 | DRA*01:01 | DRA*01:02 | DRB1*03:01 | DRB1*04:01 |
C008 | A*11:01 | A*02:01 | B*35:01 | B*27:05 | C*03:04 | C*04:01 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*04:01 | DQA1*03:03 | DQA1*01:02 | DQB1*06:04 | DQB1*03:01 | DRA*01:02 | DRA*01:01 | DRB1*13:02 | DRB1*04:01 |
C009 | A*23:01 | A*01:01 | B*13:02 | B*18:01 | C*07:01 | C*07:02 | DPA1*02:01 | DPA1*01:03 | DPB1*01:01 | DPB1*04:02 | DQA1*05:05 | DQA1*03:01 | DQB1*03:01 | DQB1*03:02 | DRA*01:01 | DRA*01:01 | DRB1*11:04 | DRB1*04:03 |
C010 | A*31:01 | A*02:06 | B*15:01 | B*56:01 | C*04:01 | C*03:03 | DPA1*02:02 | DPA1*02:02 | DPB1*05:01 | DPB1*05:01 | DQA1*03:02 | DQA1*03:02 | DQB1*03:03 | DQB1*03:96 | DRA*01:01 | DRA*01:01 | DRB1*09:01 | DRB1*09:01 |
C011 | A*02:01 | A*01:01 | B*07:02 | B*13:02 | C*06:02 | C*07:02 | DPA1*02:01 | DPA1*02:01 | DPB1*17:01 | DPB1*10:01 | DQA1*02:01 | DQA1*01:01 | DQB1*02:02 | DQB1*05:01 | DRA*01:01 | DRA*01:01 | DRB1*07:01 | DRB1*01:01 |
C012 | A*02:01 | A*02:01 | B*15:01 | B*27:02 | C*02:02 | C*03:03 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*04:01 | DQA1*01:02 | DQA1*03:01 | DQB1*03:02 | DQB1*05:02 | DRA*01:01 | DRA*01:01 | DRB1*04:04 | DRB1*16:01 |
C013 | A*02:05 | A*01:01 | B*49:01 | B*57:01 | C*07:01 | C*07:01 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*04:01 | DQA1*02:01 | DQA1*03:03 | DQB1*03:02 | DQB1*03:03 | DRA*01:01 | DRA*01:01 | DRB1*04:05 | DRB1*07:01 |
C014 | A*30:02 | A*01:01 | B*37:01 | B*27:05 | C*06:02 | C*02:02 | DPA1*02:01 | DPA1*01:03 | DPB1*02:01 | DPB1*10:01 | DQA1*03:01 | DQA1*03:03 | DQB1*04:02 | DQB1*03:02 | DRA*01:01 | DRA*01:01 | DRB1*04:03 | DRB1*04:04 |
C015 | A*02:642 | A*03:01 | B*07:02 | B*07:02 | C*07:02 | C*07:02 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*16:01 | DQA1*01:02 | DQA1*01:02 | DQB1*06:02 | DQB1*06:02 | DRA*01:02 | DRA*01:02 | DRB1*15:01 | DRB1*15:01 |
C016 | A*01:01 | A*68:01 | B*42:01 | B*08:156 | C*07:02 | C*07:01 | DPA1*01:03 | DPA1*01:03 | DPB1*04:01 | DPB1*02:01 | DQA1*05:01 | DQA1*01:02 | DQB1*02:01 | DQB1*06:02 | DRA*01:02 | DRA*01:02 | DRB1*03:01 | DRB1*15:01 |
C017 | A*03:01 | A*11:01 | B*08:01 | B*18:01 | C*05:01 | C*07:01 | DPA1*02:01 | DPA1*01:03 | DPB1*04:01 | DPB1*01:01 | DQA1*05:01 | DQA1*05:01 | DQB1*02:01 | DQB1*02:01 | DRA*01:01 | DRA*01:02 | DRB1*03:01 | DRB1*03:01 |
C018 | A*11:01 | A*02:01 | B*15:01 | B*37:01 | C*06:02 | C*04:01 | DPA1*01:03 | DPA1*02:02 | DPB1*04:01 | DPB1*05:01 | DQA1*01:03 | DQA1*01:05 | DQB1*06:01 | DQB1*05:01 | DRA*01:01 | DRA*01:02 | DRB1*10:01 | DRB1*08:03 |
C019 | A*02:10 | A*31:01 | B*54:01 | B*40:06 | C*08:01 | C*01:02 | DPA1*02:02 | DPA1*01:03 | DPB1*04:02 | DPB1*05:01 | DQA1*03:02 | DQA1*03:01 | DQB1*04:01 | DQB1*03:96 | DRA*01:01 | DRA*01:01 | DRB1*09:01 | DRB1*04:05 |
C020 | A*01:01 | A*29:02 | B*44:03 | B*08:01 | C*07:01 | C*16:01 | DPA1*02:01 | DPA1*02:02 | DPB1*01:01 | DPB1*10:01 | DQA1*02:01 | DQA1*05:01 | DQB1*02:01 | DQB1*02:02 | DRA*01:02 | DRA*01:01 | DRB1*07:01 | DRB1*03:01 |
Next, we want to check our HLA allele frequencies, and compare them to known frequencies from major populations. Here, we only include alleles with an allele frequency of 5% or higher in our study cohort. By default, MiDAS will output comparisons including the following populations, based on published data from allelefrequencies.net:
MiDAS comes with some pre-defined reference populations, but it is possible to customize these comparisons (see documentation).
allele | Counts | Freq | USA NMDP African American pop 2 | USA NMDP Chinese | USA NMDP European Caucasian | USA NMDP Hispanic South or Central American | USA NMDP Japanese | USA NMDP North American Amerindian | USA NMDP South Asian Indian |
---|---|---|---|---|---|---|---|---|---|
A*01:01 | 236 | 0.1180 | 0.0467 | 0.0145 | 0.1646 | 0.0726 | 0.0100 | 0.1202 | 0.1545 |
A*02:01 | 486 | 0.2430 | 0.1235 | 0.0946 | 0.2755 | 0.2095 | 0.1480 | 0.2776 | 0.0492 |
A*02:06 | 22 | 0.0110 | 0.0007 | 0.0349 | 0.0018 | 0.0198 | 0.0748 | 0.0275 | 0.0175 |
A*03:01 | 199 | 0.0995 | 0.0839 | 0.0140 | 0.1399 | 0.0738 | 0.0090 | 0.1044 | 0.0636 |
A*11:01 | 114 | 0.0570 | 0.0142 | 0.2752 | 0.0609 | 0.0456 | 0.0874 | 0.0488 | 0.1396 |
A*23:01 | 47 | 0.0235 | 0.1099 | 0.0021 | 0.0197 | 0.0368 | 0.0011 | 0.0181 | 0.0066 |
Let’s assume our cohort is of predominantly European ancestry. We can plot the following comparison to see if allele frequencies in our data are distributed as expected, for example by visualizing common HLA-A allele frequencies in comparison with European, Chinese, and African American reference populations:
freq_HLA_long <- tidyr::gather(
data = freq_HLA,
key = "population",
value = "freq",
"Freq",
"USA NMDP European Caucasian",
"USA NMDP Chinese",
"USA NMDP African American pop 2",
factor_key = TRUE
) %>%
filter(grepl("^A", allele))
plot_HLAfreq <-
ggplot2::ggplot(data = freq_HLA_long, ggplot2::aes(x = allele, y = freq, fill = population)) +
ggplot2::geom_bar(
stat = "identity",
position = ggplot2::position_dodge(0.7),
width = 0.7,
colour = "black"
) +
ggplot2::coord_flip() +
ggplot2::scale_y_continuous(labels = formattable::percent)
plot_HLAfreq
The following function prepares our data for analysis, combining HLA and phenotypic data into one object. Here, we are interested in analyzing our data on the level of classical HLA alleles.
We can now test our HLA data for deviations from Hardy-Weinberg-Equilibrium (HWE) to filter out alleles that strongly deviate from HWE expectations (imputation or genotyping errors, …). Here, let’s remove alleles with a HWE p-value below 0.05 divided by the number of alleles tested / present in our data (N=447). We can create a filtered MiDAS object right away (as.MiDAS = TRUE
), as done in this example, or output actual HWE test results.
HLA <- HWETest(
object = HLA,
experiment = "hla_alleles",
HWE_cutoff = 0.05 / 447,
as.MiDAS = TRUE
)
Now, we define our statistical model and run the analysis. Since we want to test for association with disease status, we use a logistic regression approach. The term
is necessary as placeholder for the tested HLA alleles and needs to be included. It will become handy when for example defining more complex interaction models.
In the runMiDAS
function, we then refer to this model, choose our analysis type and define a inheritance model. Here we use dominant model, meaning that individuals will be defined as non-carriers (0) vs. carriers (1) for a given allele. Alternatively, it is also possible to choose recessive (0 = non-carrier or heterozygous carrier, 1 = homozygous carrier), overdominant (assuming heterozygous (dis)advantage: 0 = non-carrier or homozygous carrier, 1 = heterozygous carrier), or additive (N of alleles) inheritance models. Moreover, we define allele inclusion criteria, such that we only consider alleles frequencies above 2% and below 98%. We also apply the Bonferroni method to not only get nominal P values, but also such adjusted for multiple testing. For alternative multiple testing correction methods, as well as the option to choose a custom number of tests, please refer to the documentation. exponentiate = TRUE
means that the effect estimate will already be shown as odds ratio, since we use a logistic regression model.
HLA_model <- glm(disease ~ term, data = HLA, family = binomial())
HLA_results <- runMiDAS(
object = HLA_model,
experiment = "hla_alleles",
inheritance_model = "dominant",
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
correction = "bonferroni",
exponentiate = TRUE
)
kableResults(HLA_results)
allele | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | Ntotal | Ntotal [%] | N(disease=0) | N(disease=0) [%] | N(disease=1) | N(disease=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DQB1*06:02 | 2.600e-06 | 2.290e-04 | 2.1719 | 0.1651 | 1.5764 | 3.0138 | 4.698e+00 | 198 | 9.90% | 69 | 6.90% | 129 | 12.90% |
DRB1*15:01 | 7.300e-06 | 6.386e-04 | 2.0649 | 0.1617 | 1.5081 | 2.8450 | 4.484e+00 | 206 | 10.30% | 74 | 7.40% | 132 | 13.20% |
B*57:01 | 3.080e-05 | 2.682e-03 | 2.9931 | 0.2631 | 1.8157 | 5.1189 | 4.167e+00 | 79 | 3.95% | 21 | 2.10% | 58 | 5.80% |
C*07:02 | 1.859e-04 | 1.618e-02 | 1.8227 | 0.1606 | 1.3334 | 2.5044 | 3.737e+00 | 204 | 10.20% | 78 | 7.80% | 126 | 12.60% |
B*18:01 | 6.012e-04 | 5.230e-02 | 0.5014 | 0.2012 | 0.3357 | 0.7400 | -3.431e+00 | 122 | 6.10% | 79 | 7.90% | 43 | 4.30% |
DRA*01:02 | 2.701e-03 | 2.350e-01 | 1.4703 | 0.1285 | 1.1435 | 1.8926 | 3.000e+00 | 573 | 28.65% | 263 | 26.30% | 310 | 31.00% |
DQB1*02:02 | 4.569e-03 | 3.975e-01 | 0.6257 | 0.1654 | 0.4513 | 0.8635 | -2.836e+00 | 185 | 9.25% | 110 | 11.00% | 75 | 7.50% |
B*51:01 | 4.863e-03 | 4.231e-01 | 1.7271 | 0.1941 | 1.1849 | 2.5396 | 2.816e+00 | 128 | 6.40% | 49 | 4.90% | 79 | 7.90% |
DRA*01:01 | 1.073e-02 | 9.331e-01 | 0.6210 | 0.1867 | 0.4289 | 0.8930 | -2.552e+00 | 862 | 43.10% | 445 | 44.50% | 417 | 41.70% |
B*44:02 | 2.068e-02 | 1.000e+00 | 1.4963 | 0.1742 | 1.0655 | 2.1111 | 2.314e+00 | 161 | 8.05% | 67 | 6.70% | 94 | 9.40% |
C*08:02 | 2.076e-02 | 1.000e+00 | 1.8030 | 0.2549 | 1.1023 | 3.0076 | 2.312e+00 | 71 | 3.55% | 26 | 2.60% | 45 | 4.50% |
DQA1*04:01 | 2.235e-02 | 1.000e+00 | 1.9179 | 0.2851 | 1.1089 | 3.4120 | 2.284e+00 | 57 | 2.85% | 20 | 2.00% | 37 | 3.70% |
DQB1*05:02 | 3.106e-02 | 1.000e+00 | 0.5645 | 0.2652 | 0.3313 | 0.9417 | -2.156e+00 | 65 | 3.25% | 41 | 4.10% | 24 | 2.40% |
DRB1*11:01 | 3.188e-02 | 1.000e+00 | 0.6651 | 0.1900 | 0.4565 | 0.9630 | -2.146e+00 | 131 | 6.55% | 77 | 7.70% | 54 | 5.40% |
DRB1*07:01 | 3.518e-02 | 1.000e+00 | 0.7266 | 0.1517 | 0.5390 | 0.9772 | -2.106e+00 | 228 | 11.40% | 128 | 12.80% | 100 | 10.00% |
A*31:01 | 6.065e-02 | 1.000e+00 | 1.6857 | 0.2784 | 0.9846 | 2.9492 | 1.876e+00 | 58 | 2.90% | 22 | 2.20% | 36 | 3.60% |
B*08:01 | 8.299e-02 | 1.000e+00 | 0.7130 | 0.1951 | 0.4847 | 1.0431 | -1.734e+00 | 122 | 6.10% | 70 | 7.00% | 52 | 5.20% |
DQA1*01:02 | 9.218e-02 | 1.000e+00 | 1.2555 | 0.1351 | 0.9637 | 1.6371 | 1.684e+00 | 327 | 16.35% | 151 | 15.10% | 176 | 17.60% |
B*07:02 | 1.037e-01 | 1.000e+00 | 1.3456 | 0.1825 | 0.9424 | 1.9292 | 1.627e+00 | 142 | 7.10% | 62 | 6.20% | 80 | 8.00% |
B*35:01 | 1.043e-01 | 1.000e+00 | 0.6811 | 0.2365 | 0.4254 | 1.0787 | -1.624e+00 | 80 | 4.00% | 47 | 4.70% | 33 | 3.30% |
DQB1*04:02 | 1.069e-01 | 1.000e+00 | 1.5610 | 0.2762 | 0.9141 | 2.7147 | 1.612e+00 | 58 | 2.90% | 23 | 2.30% | 35 | 3.50% |
DRB1*03:01 | 1.102e-01 | 1.000e+00 | 0.7737 | 0.1606 | 0.5639 | 1.0592 | -1.597e+00 | 194 | 9.70% | 107 | 10.70% | 87 | 8.70% |
DQA1*02:01 | 1.177e-01 | 1.000e+00 | 0.7913 | 0.1496 | 0.5895 | 1.0604 | -1.565e+00 | 235 | 11.75% | 128 | 12.80% | 107 | 10.70% |
C*03:03 | 1.291e-01 | 1.000e+00 | 0.6974 | 0.2374 | 0.4349 | 1.1070 | -1.518e+00 | 79 | 3.95% | 46 | 4.60% | 33 | 3.30% |
C*07:01 | 1.309e-01 | 1.000e+00 | 0.7952 | 0.1517 | 0.5901 | 1.0700 | -1.511e+00 | 226 | 11.30% | 123 | 12.30% | 103 | 10.30% |
DQB1*02:01 | 1.314e-01 | 1.000e+00 | 0.7860 | 0.1596 | 0.5740 | 1.0739 | -1.509e+00 | 197 | 9.85% | 108 | 10.80% | 89 | 8.90% |
DPB1*104:01 | 1.341e-01 | 1.000e+00 | 0.6295 | 0.3089 | 0.3380 | 1.1441 | -1.498e+00 | 46 | 2.30% | 28 | 2.80% | 18 | 1.80% |
DQA1*01:01 | 1.613e-01 | 1.000e+00 | 1.2610 | 0.1656 | 0.9122 | 1.7473 | 1.401e+00 | 179 | 8.95% | 81 | 8.10% | 98 | 9.80% |
DQB1*03:01 | 1.699e-01 | 1.000e+00 | 0.8355 | 0.1309 | 0.6461 | 1.0797 | -1.373e+00 | 373 | 18.65% | 197 | 19.70% | 176 | 17.60% |
A*02:01 | 1.796e-01 | 1.000e+00 | 1.1874 | 0.1280 | 0.9241 | 1.5265 | 1.342e+00 | 427 | 21.35% | 203 | 20.30% | 224 | 22.40% |
DQA1*05:05 | 2.041e-01 | 1.000e+00 | 0.8356 | 0.1414 | 0.6330 | 1.1022 | -1.270e+00 | 278 | 13.90% | 148 | 14.80% | 130 | 13.00% |
A*11:01 | 2.223e-01 | 1.000e+00 | 0.7788 | 0.2049 | 0.5194 | 1.1620 | -1.220e+00 | 108 | 5.40% | 60 | 6.00% | 48 | 4.80% |
DPA1*01:03 | 2.340e-01 | 1.000e+00 | 1.3315 | 0.2406 | 0.8330 | 2.1466 | 1.190e+00 | 924 | 46.20% | 457 | 45.70% | 467 | 46.70% |
DQB1*03:03 | 2.340e-01 | 1.000e+00 | 1.3315 | 0.2406 | 0.8330 | 2.1466 | 1.190e+00 | 76 | 3.80% | 33 | 3.30% | 43 | 4.30% |
DQA1*05:01 | 2.369e-01 | 1.000e+00 | 0.8294 | 0.1582 | 0.6076 | 1.1303 | -1.183e+00 | 201 | 10.05% | 108 | 10.80% | 93 | 9.30% |
A*25:01 | 2.563e-01 | 1.000e+00 | 0.7211 | 0.2881 | 0.4056 | 1.2630 | -1.135e+00 | 52 | 2.60% | 30 | 3.00% | 22 | 2.20% |
B*27:05 | 2.728e-01 | 1.000e+00 | 0.7374 | 0.2778 | 0.4239 | 1.2666 | -1.097e+00 | 56 | 2.80% | 32 | 3.20% | 24 | 2.40% |
DPB1*04:02 | 2.921e-01 | 1.000e+00 | 0.8426 | 0.1625 | 0.6120 | 1.1582 | -1.054e+00 | 187 | 9.35% | 100 | 10.00% | 87 | 8.70% |
A*03:01 | 2.960e-01 | 1.000e+00 | 1.1835 | 0.1612 | 0.8632 | 1.6250 | 1.045e+00 | 191 | 9.55% | 89 | 8.90% | 102 | 10.20% |
DPB1*17:01 | 2.973e-01 | 1.000e+00 | 0.7299 | 0.3020 | 0.3991 | 1.3139 | -1.042e+00 | 47 | 2.35% | 27 | 2.70% | 20 | 2.00% |
C*03:04 | 2.978e-01 | 1.000e+00 | 1.2747 | 0.2331 | 0.8086 | 2.0229 | 1.041e+00 | 81 | 4.05% | 36 | 3.60% | 45 | 4.50% |
DQA1*03:01 | 2.986e-01 | 1.000e+00 | 1.1981 | 0.1738 | 0.8526 | 1.6870 | 1.040e+00 | 158 | 7.90% | 73 | 7.30% | 85 | 8.50% |
DPB1*13:01 | 3.067e-01 | 1.000e+00 | 0.7390 | 0.2959 | 0.4093 | 1.3149 | -1.022e+00 | 49 | 2.45% | 28 | 2.80% | 21 | 2.10% |
DQA1*01:03 | 3.208e-01 | 1.000e+00 | 1.1970 | 0.1811 | 0.8398 | 1.7102 | 9.928e-01 | 143 | 7.15% | 66 | 6.60% | 77 | 7.70% |
DRB1*04:01 | 3.369e-01 | 1.000e+00 | 1.2288 | 0.2145 | 0.8079 | 1.8775 | 9.604e-01 | 97 | 4.85% | 44 | 4.40% | 53 | 5.30% |
DQB1*05:03 | 3.760e-01 | 1.000e+00 | 0.7680 | 0.2981 | 0.4239 | 1.3736 | -8.854e-01 | 48 | 2.40% | 27 | 2.70% | 21 | 2.10% |
DQA1*01:04 | 3.938e-01 | 1.000e+00 | 0.7831 | 0.2867 | 0.4426 | 1.3704 | -8.528e-01 | 52 | 2.60% | 29 | 2.90% | 23 | 2.30% |
A*32:01 | 4.177e-01 | 1.000e+00 | 0.8022 | 0.2720 | 0.4674 | 1.3647 | -8.104e-01 | 58 | 2.90% | 32 | 3.20% | 26 | 2.60% |
DQB1*06:01 | 4.558e-01 | 1.000e+00 | 1.2512 | 0.3005 | 0.6956 | 2.2759 | 7.457e-01 | 47 | 2.35% | 21 | 2.10% | 26 | 2.60% |
A*23:01 | 4.558e-01 | 1.000e+00 | 0.7993 | 0.3005 | 0.4394 | 1.4375 | -7.457e-01 | 47 | 2.35% | 26 | 2.60% | 21 | 2.10% |
B*40:01 | 4.958e-01 | 1.000e+00 | 1.2050 | 0.2738 | 0.7053 | 2.0743 | 6.812e-01 | 57 | 2.85% | 26 | 2.60% | 31 | 3.10% |
C*12:03 | 5.147e-01 | 1.000e+00 | 0.8856 | 0.1865 | 0.6135 | 1.2760 | -6.516e-01 | 133 | 6.65% | 70 | 7.00% | 63 | 6.30% |
DRB1*13:01 | 5.273e-01 | 1.000e+00 | 1.1428 | 0.2112 | 0.7557 | 1.7330 | 6.321e-01 | 100 | 5.00% | 47 | 4.70% | 53 | 5.30% |
A*29:02 | 5.379e-01 | 1.000e+00 | 0.8264 | 0.3096 | 0.4463 | 1.5145 | -6.159e-01 | 44 | 2.20% | 24 | 2.40% | 20 | 2.00% |
DRB1*01:01 | 5.622e-01 | 1.000e+00 | 0.8939 | 0.1934 | 0.6108 | 1.3059 | -5.795e-01 | 122 | 6.10% | 64 | 6.40% | 58 | 5.80% |
A*68:01 | 5.692e-01 | 1.000e+00 | 0.8499 | 0.2857 | 0.4823 | 1.4870 | -5.692e-01 | 52 | 2.60% | 28 | 2.80% | 24 | 2.40% |
B*44:03 | 5.709e-01 | 1.000e+00 | 0.8792 | 0.2271 | 0.5616 | 1.3720 | -5.667e-01 | 85 | 4.25% | 45 | 4.50% | 40 | 4.00% |
DRB1*13:02 | 5.750e-01 | 1.000e+00 | 1.1343 | 0.2247 | 0.7303 | 1.7673 | 5.607e-01 | 87 | 4.35% | 41 | 4.10% | 46 | 4.60% |
DRB1*11:04 | 5.789e-01 | 1.000e+00 | 0.8839 | 0.2224 | 0.5699 | 1.3666 | -5.550e-01 | 89 | 4.45% | 47 | 4.70% | 42 | 4.20% |
DQB1*03:02 | 6.084e-01 | 1.000e+00 | 1.0915 | 0.1709 | 0.7808 | 1.5273 | 5.123e-01 | 164 | 8.20% | 79 | 7.90% | 85 | 8.50% |
B*13:02 | 6.202e-01 | 1.000e+00 | 0.8843 | 0.2483 | 0.5415 | 1.4384 | -4.955e-01 | 70 | 3.50% | 37 | 3.70% | 33 | 3.30% |
DPA1*02:01 | 6.217e-01 | 1.000e+00 | 0.9328 | 0.1410 | 0.7072 | 1.2298 | -4.935e-01 | 279 | 13.95% | 143 | 14.30% | 136 | 13.60% |
B*15:01 | 6.412e-01 | 1.000e+00 | 0.8969 | 0.2334 | 0.5659 | 1.4173 | -4.661e-01 | 80 | 4.00% | 42 | 4.20% | 38 | 3.80% |
DRB1*04:04 | 6.542e-01 | 1.000e+00 | 0.8745 | 0.2994 | 0.4829 | 1.5731 | -4.480e-01 | 47 | 2.35% | 25 | 2.50% | 22 | 2.20% |
B*38:01 | 6.665e-01 | 1.000e+00 | 0.8833 | 0.2880 | 0.4993 | 1.5537 | -4.310e-01 | 51 | 2.55% | 27 | 2.70% | 24 | 2.40% |
DQB1*06:04 | 7.083e-01 | 1.000e+00 | 1.0980 | 0.2498 | 0.6727 | 1.7976 | 3.742e-01 | 69 | 3.45% | 33 | 3.30% | 36 | 3.60% |
DPB1*03:01 | 7.087e-01 | 1.000e+00 | 0.9325 | 0.1869 | 0.6457 | 1.3454 | -3.736e-01 | 132 | 6.60% | 68 | 6.80% | 64 | 6.40% |
DPB1*02:01 | 7.233e-01 | 1.000e+00 | 0.9511 | 0.1417 | 0.7203 | 1.2555 | -3.541e-01 | 275 | 13.75% | 140 | 14.00% | 135 | 13.50% |
C*12:02 | 7.526e-01 | 1.000e+00 | 0.9053 | 0.3156 | 0.4842 | 1.6832 | -3.152e-01 | 42 | 2.10% | 22 | 2.20% | 20 | 2.00% |
A*24:02 | 7.586e-01 | 1.000e+00 | 1.0484 | 0.1537 | 0.7756 | 1.4176 | 3.074e-01 | 216 | 10.80% | 106 | 10.60% | 110 | 11.00% |
C*05:01 | 7.899e-01 | 1.000e+00 | 0.9538 | 0.1776 | 0.6728 | 1.3513 | -2.664e-01 | 149 | 7.45% | 76 | 7.60% | 73 | 7.30% |
DPB1*01:01 | 8.067e-01 | 1.000e+00 | 0.9419 | 0.2448 | 0.5814 | 1.5231 | -2.446e-01 | 72 | 3.60% | 37 | 3.70% | 35 | 3.50% |
C*02:02 | 8.215e-01 | 1.000e+00 | 1.0522 | 0.2256 | 0.6756 | 1.6408 | 2.256e-01 | 86 | 4.30% | 42 | 4.20% | 44 | 4.40% |
C*01:02 | 8.316e-01 | 1.000e+00 | 1.0463 | 0.2128 | 0.6891 | 1.5902 | 2.127e-01 | 98 | 4.90% | 48 | 4.80% | 50 | 5.00% |
DQB1*06:03 | 8.385e-01 | 1.000e+00 | 1.0424 | 0.2038 | 0.6987 | 1.5563 | 2.038e-01 | 108 | 5.40% | 53 | 5.30% | 55 | 5.50% |
DQB1*05:01 | 8.724e-01 | 1.000e+00 | 1.0261 | 0.1606 | 0.7489 | 1.4063 | 1.606e-01 | 192 | 9.60% | 95 | 9.50% | 97 | 9.70% |
A*30:02 | 8.761e-01 | 1.000e+00 | 0.9526 | 0.3119 | 0.5141 | 1.7603 | -1.559e-01 | 43 | 2.15% | 22 | 2.20% | 21 | 2.10% |
DRB1*04:05 | 8.812e-01 | 1.000e+00 | 1.0457 | 0.2989 | 0.5806 | 1.8875 | 1.494e-01 | 47 | 2.35% | 23 | 2.30% | 24 | 2.40% |
C*15:02 | 8.812e-01 | 1.000e+00 | 0.9563 | 0.2989 | 0.5298 | 1.7224 | -1.494e-01 | 47 | 2.35% | 24 | 2.40% | 23 | 2.30% |
C*16:01 | 8.835e-01 | 1.000e+00 | 0.9580 | 0.2930 | 0.5370 | 1.7054 | -1.465e-01 | 49 | 2.45% | 25 | 2.50% | 24 | 2.40% |
DQA1*03:03 | 9.248e-01 | 1.000e+00 | 0.9824 | 0.1887 | 0.6781 | 1.4227 | -9.434e-02 | 129 | 6.45% | 65 | 6.50% | 64 | 6.40% |
C*06:02 | 9.348e-01 | 1.000e+00 | 0.9867 | 0.1636 | 0.7158 | 1.3601 | -8.178e-02 | 183 | 9.15% | 92 | 9.20% | 91 | 9.10% |
C*04:01 | 9.396e-01 | 1.000e+00 | 1.0115 | 0.1515 | 0.7516 | 1.3615 | 7.573e-02 | 225 | 11.25% | 112 | 11.20% | 113 | 11.30% |
A*26:01 | 1.000e+00 | 1.000e+00 | 1.0000 | 0.2232 | 0.6447 | 1.5510 | 0.000e+00 | 88 | 4.40% | 44 | 4.40% | 44 | 4.40% |
B*52:01 | 1.000e+00 | 1.000e+00 | 1.0000 | 0.3153 | 0.5367 | 1.8633 | 0.000e+00 | 42 | 2.10% | 21 | 2.10% | 21 | 2.10% |
A*01:01 | 1.000e+00 | 1.000e+00 | 1.0000 | 0.1522 | 0.7419 | 1.3479 | 0.000e+00 | 222 | 11.10% | 111 | 11.10% | 111 | 11.10% |
B*49:01 | 1.000e+00 | 1.000e+00 | 1.0000 | 0.3153 | 0.5367 | 1.8633 | 0.000e+00 | 42 | 2.10% | 21 | 2.10% | 21 | 2.10% |
Three HLA alleles show significant association with the disease after multiple testing adjustment. Due to the complex linkage disequilibrium structure in the MHC, it is likely that associations are not statistically independent. The two alleles HLA-DRB1*15:01 and HLA-DQB1*06:02 are a common class II haplotype. We can therefore test if there are associations that are statistically independent of our top-associated allele, by setting the conditional
flag to TRUE
. MiDAS will now perform stepwise conditional testing until the top associated allele does not reach a defined significance threshold (here th = 0.05
, based on adjusted p value).
HLA_results_cond <- runMiDAS(
object = HLA_model,
experiment = "hla_alleles",
inheritance_model = "dominant",
conditional = TRUE,
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
correction = "bonferroni",
exponentiate = TRUE
)
kableResults(HLA_results_cond, scroll_box_height = "200px")
allele | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | covariates | Ntotal | Ntotal [%] | N(disease=0) | N(disease=0) [%] | N(disease=1) | N(disease=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DQB1*06:02 | 2.60e-06 | 2.290e-04 | 2.172 | 0.1651 | 1.576 | 3.014 | 4.698 | 198 | 9.90% | 69 | 6.90% | 129 | 12.90% | |
B*57:01 | 2.64e-05 | 2.269e-03 | 3.049 | 0.2653 | 1.841 | 5.235 | 4.203 | DQB1*06:02 | 79 | 3.95% | 21 | 2.10% | 58 | 5.80% |
The results for conditional testing are displayed in a way that for each step the top associated allele is shown, along with a list of alleles conditioned on.
As we can see, HLA-DRB1*15:01 was not independently associated with the disease when correcting for our top-associated allele HLA-DQB1*06:02. However, HLA-B*57:01 can be considered an independent association signal.
Next, we want to find out what are the strongest associated amino acid positions, corresponding to our allele-level associations. This can help fine-mapping the associated variants to e.g. the peptide binding region or other functionally distinct parts of the protein. We thus prepare a MiDAS object with experiment type “hla_aa”, which includes the inference of amino acid variation from allele calls.
Amino acid data will be stored in a MiDAS object, but we can extract it to a data frame and select a couple of variables to display how this looks like:
dat_HLA_AA <- HLA_AA[["hla_aa"]] %>%
assay() %>%
t() %>%
as.data.frame() %>%
select(starts_with("B_97_")) %>%
head()
B_97_S | B_97_R | B_97_T | B_97_N | B_97_V | B_97_W | |
---|---|---|---|---|---|---|
C001 | 1 | 1 | 0 | 0 | 0 | 0 |
C002 | 0 | 2 | 0 | 0 | 0 | 0 |
C003 | 0 | 1 | 1 | 0 | 0 | 0 |
C004 | 1 | 1 | 0 | 0 | 0 | 0 |
C005 | 1 | 1 | 0 | 0 | 0 | 0 |
C006 | 2 | 0 | 0 | 0 | 0 | 0 |
Now, we run the association test based on amino acid variation. To first identify the most relevant associated amino acid positions, we run a likelihood ratio (omnibus) test, which groups all residues at each amino acid position.
HLA_AA_model <- glm(disease ~ term, data = HLA_AA, family = binomial())
HLA_AA_omnibus_results <- runMiDAS(
HLA_AA_model,
experiment = "hla_aa",
inheritance_model = "dominant",
conditional = FALSE,
omnibus = TRUE,
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
correction = "bonferroni"
)
kableResults(HLA_AA_omnibus_results)
aa_pos | residues | df | statistic | p.value | p.adjusted |
---|---|---|---|---|---|
B_97 | S, R, T, N, V, W | 6 | 3.532e+01 | 3.700e-06 | 1.573e-03 |
DQB1_9 | F, Y, * | 3 | 2.569e+01 | 1.110e-05 | 4.682e-03 |
DQB1_185 | *, T | 2 | 1.881e+01 | 8.240e-05 | 3.476e-02 |
DQB1_177 | *, H | 2 | 1.788e+01 | 1.313e-04 | 5.542e-02 |
DQB1_142 | *, V | 2 | 1.788e+01 | 1.313e-04 | 5.542e-02 |
DQB1_167 | *, R | 2 | 1.729e+01 | 1.759e-04 | 7.422e-02 |
DQB1_116 | *, V | 2 | 1.695e+01 | 2.081e-04 | 8.782e-02 |
DQB1_125 | *, A | 2 | 1.695e+01 | 2.081e-04 | 8.782e-02 |
B_81 | L, A | 2 | 1.689e+01 | 2.154e-04 | 9.092e-02 |
DQB1_130 | *, R | 2 | 1.663e+01 | 2.451e-04 | 1.034e-01 |
DQB1_126 | *, Q | 2 | 1.636e+01 | 2.807e-04 | 1.185e-01 |
B_80 | N, T, I | 3 | 1.866e+01 | 3.209e-04 | 1.354e-01 |
B_82 | R, L | 2 | 1.582e+01 | 3.674e-04 | 1.550e-01 |
B_83 | G, R | 2 | 1.582e+01 | 3.674e-04 | 1.550e-01 |
DQB1_140 | *, A, T | 3 | 1.836e+01 | 3.711e-04 | 1.566e-01 |
DQB1_182 | *, S, N | 3 | 1.836e+01 | 3.711e-04 | 1.566e-01 |
DQB1_135 | *, G, D | 3 | 1.815e+01 | 4.087e-04 | 1.725e-01 |
B_62 | R, G | 2 | 1.511e+01 | 5.232e-04 | 2.208e-01 |
DRB1_71 | A, R, E, K | 4 | 1.984e+01 | 5.362e-04 | 2.263e-01 |
B_275 | *, E | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_295 | *, . | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_296 | *, A | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_297 | *, V | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_299 | *, V | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_300 | *, I | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_301 | *, G | 2 | 1.476e+01 | 6.231e-04 | 2.630e-01 |
B_77 | S, N, D | 3 | 1.717e+01 | 6.530e-04 | 2.756e-01 |
B_65 | Q, R | 2 | 1.385e+01 | 9.828e-04 | 4.147e-01 |
B_70 | Q, N, K, S | 4 | 1.849e+01 | 9.909e-04 | 4.181e-01 |
B_239 | *, R | 2 | 1.362e+01 | 1.105e-03 | 4.664e-01 |
B_253 | *, E | 2 | 1.362e+01 | 1.105e-03 | 4.664e-01 |
B_267 | *, P | 2 | 1.362e+01 | 1.105e-03 | 4.664e-01 |
B_268 | *, K | 2 | 1.362e+01 | 1.105e-03 | 4.664e-01 |
B_270 | *, L | 2 | 1.362e+01 | 1.105e-03 | 4.664e-01 |
B_66 | I, N | 2 | 1.330e+01 | 1.294e-03 | 5.460e-01 |
B_194 | *, I | 2 | 1.312e+01 | 1.415e-03 | 5.970e-01 |
B_103 | V, L | 2 | 1.296e+01 | 1.537e-03 | 6.484e-01 |
B_30 | D, G | 2 | 1.283e+01 | 1.637e-03 | 6.907e-01 |
B_-16 | *, V | 2 | 1.255e+01 | 1.886e-03 | 7.960e-01 |
B_339 | *, A | 2 | 1.242e+01 | 2.010e-03 | 8.481e-01 |
B_199 | *, A | 2 | 1.215e+01 | 2.298e-03 | 9.696e-01 |
B_211 | *, A | 2 | 1.207e+01 | 2.395e-03 | 1.000e+00 |
DQB1_55 | R, L, P | 3 | 1.420e+01 | 2.647e-03 | 1.000e+00 |
C_99 | Y, C, F, S | 4 | 1.624e+01 | 2.714e-03 | 1.000e+00 |
DRA_217 | L, V | 2 | 1.173e+01 | 2.830e-03 | 1.000e+00 |
B_9 | Y, H, D | 3 | 1.370e+01 | 3.340e-03 | 1.000e+00 |
B_69 | A, T | 2 | 1.122e+01 | 3.661e-03 | 1.000e+00 |
DQB1_38 | A, V | 2 | 1.081e+01 | 4.489e-03 | 1.000e+00 |
DQB1_77 | T, R, * | 3 | 1.287e+01 | 4.925e-03 | 1.000e+00 |
B_67 | Y, S, F, C, M | 5 | 1.666e+01 | 5.181e-03 | 1.000e+00 |
B_71 | A, T | 2 | 1.044e+01 | 5.405e-03 | 1.000e+00 |
DQB1_87 | F, L, *, Y | 4 | 1.424e+01 | 6.568e-03 | 1.000e+00 |
DQB1_30 | Y, S, H | 3 | 1.148e+01 | 9.382e-03 | 1.000e+00 |
B_-23 | *, R | 2 | 9.202e+00 | 1.004e-02 | 1.000e+00 |
B_-21 | *, T | 2 | 9.202e+00 | 1.004e-02 | 1.000e+00 |
DQB1_28 | T, S | 2 | 9.008e+00 | 1.106e-02 | 1.000e+00 |
DQB1_46 | V, E | 2 | 9.008e+00 | 1.106e-02 | 1.000e+00 |
DQB1_47 | Y, F | 2 | 9.008e+00 | 1.106e-02 | 1.000e+00 |
DQB1_52 | P, L | 2 | 9.008e+00 | 1.106e-02 | 1.000e+00 |
B_-10 | *, G | 2 | 8.724e+00 | 1.275e-02 | 1.000e+00 |
B_-9 | *, A | 2 | 8.473e+00 | 1.446e-02 | 1.000e+00 |
DQB1_74 | E, A, S, * | 4 | 1.222e+01 | 1.579e-02 | 1.000e+00 |
B_163 | E, L, T | 3 | 1.031e+01 | 1.610e-02 | 1.000e+00 |
B_282 | * | 1 | 5.561e+00 | 1.836e-02 | 1.000e+00 |
B_306 | * | 1 | 5.561e+00 | 1.836e-02 | 1.000e+00 |
B_326 | * | 1 | 5.561e+00 | 1.836e-02 | 1.000e+00 |
DQA1_107 | I, *, T | 3 | 9.899e+00 | 1.945e-02 | 1.000e+00 |
DQA1_156 | L, *, F | 3 | 9.899e+00 | 1.945e-02 | 1.000e+00 |
DQA1_161 | E, *, D | 3 | 9.899e+00 | 1.945e-02 | 1.000e+00 |
DQA1_163 | S, *, I | 3 | 9.899e+00 | 1.945e-02 | 1.000e+00 |
DQB1_71 | T, K, D, A, * | 5 | 1.324e+01 | 2.121e-02 | 1.000e+00 |
DQB1_57 | D, A, V, S | 4 | 1.140e+01 | 2.237e-02 | 1.000e+00 |
DQB1_37 | Y, I, D | 3 | 9.592e+00 | 2.237e-02 | 1.000e+00 |
DRB1_25 | R, Q | 2 | 7.501e+00 | 2.350e-02 | 1.000e+00 |
DRB1_14 | E, K | 2 | 7.447e+00 | 2.415e-02 | 1.000e+00 |
DQA1_175 | K, *, Q, E | 4 | 1.122e+01 | 2.421e-02 | 1.000e+00 |
DRB1_13 | R, Y, S, F, H, G | 6 | 1.442e+01 | 2.532e-02 | 1.000e+00 |
DQA1_75 | S, I | 2 | 7.203e+00 | 2.729e-02 | 1.000e+00 |
DQB1_75 | L, V, * | 3 | 9.131e+00 | 2.760e-02 | 1.000e+00 |
DPB1_85 | G, E | 2 | 7.165e+00 | 2.781e-02 | 1.000e+00 |
DPB1_86 | P, A | 2 | 7.165e+00 | 2.781e-02 | 1.000e+00 |
DPB1_87 | M, V | 2 | 7.165e+00 | 2.781e-02 | 1.000e+00 |
DQA1_-16 | M, *, L | 3 | 8.964e+00 | 2.977e-02 | 1.000e+00 |
DRB1_11 | P, G, S, V, L | 5 | 1.229e+01 | 3.107e-02 | 1.000e+00 |
B_-8 | *, V | 2 | 6.935e+00 | 3.120e-02 | 1.000e+00 |
DQA1_69 | L, A, T | 3 | 8.813e+00 | 3.189e-02 | 1.000e+00 |
DPB1_56 | E, A | 2 | 6.804e+00 | 3.331e-02 | 1.000e+00 |
B_-11 | *, W | 2 | 6.510e+00 | 3.858e-02 | 1.000e+00 |
DRB1_73 | A, G | 2 | 6.484e+00 | 3.909e-02 | 1.000e+00 |
DQA1_56 | ., G, R | 3 | 8.283e+00 | 4.051e-02 | 1.000e+00 |
DQA1_76 | L, M, V | 3 | 8.283e+00 | 4.051e-02 | 1.000e+00 |
B_63 | N, E | 2 | 6.264e+00 | 4.363e-02 | 1.000e+00 |
B_143 | T, S | 2 | 6.242e+00 | 4.412e-02 | 1.000e+00 |
B_147 | W, L | 2 | 6.242e+00 | 4.412e-02 | 1.000e+00 |
DQB1_53 | Q, L | 2 | 6.167e+00 | 4.581e-02 | 1.000e+00 |
DRB1_70 | Q, D, R | 3 | 7.952e+00 | 4.701e-02 | 1.000e+00 |
B_178 | K, T | 2 | 6.097e+00 | 4.744e-02 | 1.000e+00 |
DQA1_129 | H, *, Q | 3 | 7.675e+00 | 5.323e-02 | 1.000e+00 |
DRB1_78 | Y, V | 2 | 5.806e+00 | 5.486e-02 | 1.000e+00 |
B_45 | E, K, M, T | 4 | 9.174e+00 | 5.689e-02 | 1.000e+00 |
B_152 | E, V | 2 | 5.717e+00 | 5.736e-02 | 1.000e+00 |
A_156 | W, L, Q, R | 4 | 9.095e+00 | 5.876e-02 | 1.000e+00 |
DPB1_84 | G, D | 2 | 5.518e+00 | 6.335e-02 | 1.000e+00 |
DQA1_47 | C, K, R, Q | 4 | 8.866e+00 | 6.454e-02 | 1.000e+00 |
B_167 | W, S | 2 | 5.462e+00 | 6.514e-02 | 1.000e+00 |
DPB1_69 | E, K, R | 3 | 7.206e+00 | 6.560e-02 | 1.000e+00 |
DRB1_96 | *, H | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_98 | *, K | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_104 | *, S | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_120 | *, S | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_149 | *, H | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_180 | *, V | 2 | 5.296e+00 | 7.080e-02 | 1.000e+00 |
DRB1_58 | A, E | 2 | 5.167e+00 | 7.550e-02 | 1.000e+00 |
DQA1_54 | F, L | 2 | 4.945e+00 | 8.436e-02 | 1.000e+00 |
DQB1_56 | P, L | 2 | 4.874e+00 | 8.743e-02 | 1.000e+00 |
DRB1_74 | A, Q, E, R, L | 5 | 9.459e+00 | 9.208e-02 | 1.000e+00 |
B_116 | Y, L, S, F, D | 5 | 9.438e+00 | 9.281e-02 | 1.000e+00 |
DRB1_77 | T, N | 2 | 4.705e+00 | 9.511e-02 | 1.000e+00 |
DQB1_66 | E, D | 2 | 4.696e+00 | 9.554e-02 | 1.000e+00 |
DQB1_67 | V, I | 2 | 4.696e+00 | 9.554e-02 | 1.000e+00 |
DQB1_23 | R | 1 | 2.777e+00 | 9.565e-02 | 1.000e+00 |
B_46 | E, A | 2 | 4.545e+00 | 1.030e-01 | 1.000e+00 |
DQA1_18 | S, F | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_45 | V, A | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_48 | L, W | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_55 | R, G | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_61 | F, G | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_64 | T, R | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_66 | I, M | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
DQA1_80 | S, Y | 2 | 4.503e+00 | 1.052e-01 | 1.000e+00 |
A_163 | R, T | 2 | 4.467e+00 | 1.072e-01 | 1.000e+00 |
DQA1_11 | Y, C | 2 | 4.452e+00 | 1.080e-01 | 1.000e+00 |
A_90 | D, A | 2 | 4.340e+00 | 1.142e-01 | 1.000e+00 |
DPA1_31 | M, Q | 2 | 4.297e+00 | 1.167e-01 | 1.000e+00 |
DPA1_50 | Q, R | 2 | 4.237e+00 | 1.202e-01 | 1.000e+00 |
A_245 | *, A | 2 | 4.225e+00 | 1.210e-01 | 1.000e+00 |
B_171 | Y, H | 2 | 4.132e+00 | 1.267e-01 | 1.000e+00 |
DQB1_84 | E, Q, * | 3 | 5.693e+00 | 1.276e-01 | 1.000e+00 |
DQB1_85 | V, L, * | 3 | 5.693e+00 | 1.276e-01 | 1.000e+00 |
DQB1_89 | G, T, * | 3 | 5.693e+00 | 1.276e-01 | 1.000e+00 |
DQB1_90 | I, T, * | 3 | 5.693e+00 | 1.276e-01 | 1.000e+00 |
DPB1_36 | V, A | 2 | 4.077e+00 | 1.302e-01 | 1.000e+00 |
DQB1_70 | G, R, E, * | 4 | 7.108e+00 | 1.303e-01 | 1.000e+00 |
DQA1_130 | S, *, A | 3 | 5.630e+00 | 1.311e-01 | 1.000e+00 |
DRB1_37 | S, F, N, Y, L | 5 | 8.451e+00 | 1.331e-01 | 1.000e+00 |
DQA1_102 | L, * | 2 | 4.014e+00 | 1.344e-01 | 1.000e+00 |
DQA1_138 | T, * | 2 | 4.014e+00 | 1.344e-01 | 1.000e+00 |
DQA1_139 | S, * | 2 | 4.014e+00 | 1.344e-01 | 1.000e+00 |
DRB1_10 | Q, Y | 2 | 3.965e+00 | 1.377e-01 | 1.000e+00 |
DQA1_50 | V, L, E | 3 | 5.421e+00 | 1.435e-01 | 1.000e+00 |
DQA1_53 | Q, R, K | 3 | 5.421e+00 | 1.435e-01 | 1.000e+00 |
DQA1_52 | R, H, S | 3 | 5.388e+00 | 1.455e-01 | 1.000e+00 |
A_105 | P, S | 2 | 3.819e+00 | 1.482e-01 | 1.000e+00 |
A_193 | *, A | 2 | 3.707e+00 | 1.567e-01 | 1.000e+00 |
A_194 | *, V | 2 | 3.707e+00 | 1.567e-01 | 1.000e+00 |
A_207 | *, S | 2 | 3.707e+00 | 1.567e-01 | 1.000e+00 |
A_253 | *, Q | 2 | 3.707e+00 | 1.567e-01 | 1.000e+00 |
DQA1_-13 | T, *, A | 3 | 5.123e+00 | 1.630e-01 | 1.000e+00 |
DPA1_83 | T, A | 2 | 3.592e+00 | 1.660e-01 | 1.000e+00 |
A_77 | S, D, N | 3 | 4.931e+00 | 1.770e-01 | 1.000e+00 |
DRB1_12 | K, T | 2 | 3.424e+00 | 1.805e-01 | 1.000e+00 |
C_66 | K, N | 2 | 3.422e+00 | 1.807e-01 | 1.000e+00 |
DRB1_60 | Y, S, H | 3 | 4.810e+00 | 1.863e-01 | 1.000e+00 |
DQB1_45 | G, E | 2 | 3.314e+00 | 1.907e-01 | 1.000e+00 |
DRB1_30 | Y, L, C, H | 4 | 6.083e+00 | 1.930e-01 | 1.000e+00 |
DQA1_40 | G, E | 2 | 3.267e+00 | 1.953e-01 | 1.000e+00 |
DQA1_51 | L, F | 2 | 3.267e+00 | 1.953e-01 | 1.000e+00 |
C_152 | E, A | 2 | 3.198e+00 | 2.021e-01 | 1.000e+00 |
C_77 | S, N | 2 | 3.197e+00 | 2.022e-01 | 1.000e+00 |
C_80 | N, K | 2 | 3.197e+00 | 2.022e-01 | 1.000e+00 |
DPA1_96 | *, P | 1 | 1.600e+00 | 2.059e-01 | 1.000e+00 |
B_113 | H, Y | 2 | 3.079e+00 | 2.145e-01 | 1.000e+00 |
A_73 | T, I | 2 | 3.047e+00 | 2.180e-01 | 1.000e+00 |
DRB1_28 | D, E | 2 | 3.036e+00 | 2.192e-01 | 1.000e+00 |
DQA1_160 | A, *, D | 3 | 4.381e+00 | 2.231e-01 | 1.000e+00 |
DPB1_55 | D, E, A | 3 | 4.338e+00 | 2.272e-01 | 1.000e+00 |
DRB1_33 | N, H | 2 | 2.928e+00 | 2.313e-01 | 1.000e+00 |
DQA1_1 | E, * | 2 | 2.925e+00 | 2.317e-01 | 1.000e+00 |
DQA1_218 | R, *, Q | 3 | 4.267e+00 | 2.340e-01 | 1.000e+00 |
DQA1_41 | R, K | 2 | 2.893e+00 | 2.354e-01 | 1.000e+00 |
A_43 | Q | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
B_109 | L | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_-17 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_-15 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_-9 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_1 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_175 | G | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_184 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_219 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_253 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_267 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_275 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_285 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_312 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
C_345 | * | 1 | 1.387e+00 | 2.389e-01 | 1.000e+00 |
DQB1_86 | A, E, *, G | 4 | 5.435e+00 | 2.455e-01 | 1.000e+00 |
DQA1_2 | D, *, G | 3 | 4.104e+00 | 2.505e-01 | 1.000e+00 |
C_173 | E, K | 2 | 2.722e+00 | 2.564e-01 | 1.000e+00 |
A_219 | *, R | 2 | 2.718e+00 | 2.569e-01 | 1.000e+00 |
C_103 | L, V | 2 | 2.700e+00 | 2.592e-01 | 1.000e+00 |
C_91 | G, R | 2 | 2.649e+00 | 2.659e-01 | 1.000e+00 |
DRB1_16 | H, Y | 2 | 2.631e+00 | 2.684e-01 | 1.000e+00 |
DPB1_-14 | *, T | 2 | 2.558e+00 | 2.782e-01 | 1.000e+00 |
DRB1_57 | D, V, A, S | 4 | 5.068e+00 | 2.804e-01 | 1.000e+00 |
DQA1_26 | T, S | 2 | 2.541e+00 | 2.807e-01 | 1.000e+00 |
A_282 | *, I | 2 | 2.520e+00 | 2.836e-01 | 1.000e+00 |
A_311 | *, K | 2 | 2.520e+00 | 2.836e-01 | 1.000e+00 |
C_330 | *, A | 2 | 2.517e+00 | 2.840e-01 | 1.000e+00 |
DRB1_32 | Y, H | 2 | 2.498e+00 | 2.868e-01 | 1.000e+00 |
B_94 | T, I | 2 | 2.473e+00 | 2.904e-01 | 1.000e+00 |
DQB1_13 | G, A, * | 3 | 3.735e+00 | 2.915e-01 | 1.000e+00 |
C_24 | A, S | 2 | 2.431e+00 | 2.966e-01 | 1.000e+00 |
C_295 | *, A | 2 | 2.316e+00 | 3.142e-01 | 1.000e+00 |
C_311 | *, A | 2 | 2.316e+00 | 3.142e-01 | 1.000e+00 |
C_313 | *, V | 2 | 2.316e+00 | 3.142e-01 | 1.000e+00 |
C_332 | *, S | 2 | 2.316e+00 | 3.142e-01 | 1.000e+00 |
DRB1_47 | F, Y | 2 | 2.287e+00 | 3.187e-01 | 1.000e+00 |
A_9 | Y, F, S, T | 4 | 4.680e+00 | 3.218e-01 | 1.000e+00 |
B_12 | V, M | 2 | 2.186e+00 | 3.352e-01 | 1.000e+00 |
DRB1_67 | I, L, F | 3 | 3.360e+00 | 3.394e-01 | 1.000e+00 |
B_11 | S, A | 2 | 2.145e+00 | 3.421e-01 | 1.000e+00 |
DQA1_207 | V, *, M | 3 | 3.336e+00 | 3.426e-01 | 1.000e+00 |
DPB1_11 | G, L | 2 | 2.127e+00 | 3.452e-01 | 1.000e+00 |
C_113 | Y, H | 2 | 2.014e+00 | 3.653e-01 | 1.000e+00 |
A_109 | L | 1 | 8.077e-01 | 3.688e-01 | 1.000e+00 |
A_231 | *, V | 2 | 1.979e+00 | 3.718e-01 | 1.000e+00 |
B_145 | R, L | 2 | 1.976e+00 | 3.723e-01 | 1.000e+00 |
DPB1_8 | L, V | 2 | 1.958e+00 | 3.758e-01 | 1.000e+00 |
C_309 | *, V | 2 | 1.876e+00 | 3.915e-01 | 1.000e+00 |
C_327 | *, C | 2 | 1.876e+00 | 3.915e-01 | 1.000e+00 |
C_14 | R, W | 2 | 1.834e+00 | 3.998e-01 | 1.000e+00 |
C_49 | A, E | 2 | 1.834e+00 | 3.998e-01 | 1.000e+00 |
A_76 | E, V, A | 3 | 2.935e+00 | 4.018e-01 | 1.000e+00 |
DPB1_178 | *, L | 2 | 1.820e+00 | 4.026e-01 | 1.000e+00 |
B_32 | Q, L | 2 | 1.766e+00 | 4.136e-01 | 1.000e+00 |
DQB1_14 | M, L, * | 3 | 2.820e+00 | 4.202e-01 | 1.000e+00 |
A_144 | Q, K | 2 | 1.719e+00 | 4.234e-01 | 1.000e+00 |
DRB1_9 | W, E | 2 | 1.605e+00 | 4.482e-01 | 1.000e+00 |
C_194 | *, V | 2 | 1.600e+00 | 4.493e-01 | 1.000e+00 |
C_261 | *, V | 2 | 1.600e+00 | 4.493e-01 | 1.000e+00 |
C_273 | *, R | 2 | 1.600e+00 | 4.493e-01 | 1.000e+00 |
C_114 | D, N | 2 | 1.576e+00 | 4.547e-01 | 1.000e+00 |
C_248 | *, V | 2 | 1.560e+00 | 4.583e-01 | 1.000e+00 |
C_211 | *, A | 2 | 1.554e+00 | 4.597e-01 | 1.000e+00 |
DPB1_76 | M, V, I | 3 | 2.568e+00 | 4.631e-01 | 1.000e+00 |
C_147 | W, L | 2 | 1.511e+00 | 4.697e-01 | 1.000e+00 |
C_35 | R, Q | 2 | 1.502e+00 | 4.718e-01 | 1.000e+00 |
C_-18 | *, R | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_-5 | *, T | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_-1 | *, A | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_284 | *, I | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_289 | *, A | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_291 | *, L | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_314 | *, M | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
C_315 | *, C | 2 | 1.478e+00 | 4.775e-01 | 1.000e+00 |
DPB1_33 | Q | 1 | 4.988e-01 | 4.800e-01 | 1.000e+00 |
DQA1_208 | G, * | 2 | 1.451e+00 | 4.842e-01 | 1.000e+00 |
DRB1_86 | V, G | 2 | 1.448e+00 | 4.847e-01 | 1.000e+00 |
DQA1_-7 | V, *, M | 3 | 2.441e+00 | 4.860e-01 | 1.000e+00 |
A_297 | *, V | 2 | 1.434e+00 | 4.881e-01 | 1.000e+00 |
C_270 | *, L | 2 | 1.412e+00 | 4.937e-01 | 1.000e+00 |
A_186 | *, K | 2 | 1.397e+00 | 4.973e-01 | 1.000e+00 |
A_-16 | *, L | 2 | 1.395e+00 | 4.979e-01 | 1.000e+00 |
A_283 | *, P | 2 | 1.395e+00 | 4.979e-01 | 1.000e+00 |
C_21 | R, H | 2 | 1.394e+00 | 4.980e-01 | 1.000e+00 |
C_186 | *, K | 2 | 1.387e+00 | 4.997e-01 | 1.000e+00 |
C_310 | *, V | 2 | 1.387e+00 | 4.997e-01 | 1.000e+00 |
A_184 | *, A, P | 3 | 2.353e+00 | 5.024e-01 | 1.000e+00 |
C_73 | A, T | 2 | 1.376e+00 | 5.027e-01 | 1.000e+00 |
C_163 | T, L, E | 3 | 2.260e+00 | 5.203e-01 | 1.000e+00 |
DPA1_11 | A, M | 2 | 1.299e+00 | 5.224e-01 | 1.000e+00 |
A_149 | T, A | 2 | 1.254e+00 | 5.343e-01 | 1.000e+00 |
A_56 | G, R | 2 | 1.228e+00 | 5.411e-01 | 1.000e+00 |
B_41 | A, T | 2 | 1.228e+00 | 5.412e-01 | 1.000e+00 |
A_161 | E, D | 2 | 1.204e+00 | 5.478e-01 | 1.000e+00 |
DPB1_9 | F, Y, H | 3 | 2.109e+00 | 5.501e-01 | 1.000e+00 |
C_138 | T, K | 2 | 1.169e+00 | 5.573e-01 | 1.000e+00 |
A_151 | H, R | 2 | 1.161e+00 | 5.597e-01 | 1.000e+00 |
DQA1_199 | A, *, T | 3 | 2.025e+00 | 5.672e-01 | 1.000e+00 |
DPB1_194 | *, R | 2 | 1.111e+00 | 5.737e-01 | 1.000e+00 |
DPB1_215 | *, I | 2 | 1.111e+00 | 5.737e-01 | 1.000e+00 |
B_131 | R, S | 2 | 1.054e+00 | 5.902e-01 | 1.000e+00 |
A_107 | G, W | 2 | 1.047e+00 | 5.926e-01 | 1.000e+00 |
C_94 | T, I | 2 | 1.044e+00 | 5.935e-01 | 1.000e+00 |
A_63 | N, E, Q | 3 | 1.882e+00 | 5.973e-01 | 1.000e+00 |
DQA1_-6 | M, * | 2 | 1.031e+00 | 5.973e-01 | 1.000e+00 |
DRB1_26 | F, Y, L | 3 | 1.844e+00 | 6.054e-01 | 1.000e+00 |
A_74 | D, H | 2 | 1.002e+00 | 6.059e-01 | 1.000e+00 |
DQA1_187 | A, *, T | 3 | 1.819e+00 | 6.109e-01 | 1.000e+00 |
DQA1_215 | F, *, L | 3 | 1.819e+00 | 6.109e-01 | 1.000e+00 |
C_90 | A, D | 2 | 9.661e-01 | 6.169e-01 | 1.000e+00 |
C_95 | L, I | 2 | 9.617e-01 | 6.183e-01 | 1.000e+00 |
C_11 | A, S | 2 | 9.525e-01 | 6.211e-01 | 1.000e+00 |
B_158 | A, T | 2 | 9.012e-01 | 6.372e-01 | 1.000e+00 |
B_114 | D, N, H | 3 | 1.682e+00 | 6.410e-01 | 1.000e+00 |
A_-18 | *, R | 2 | 8.755e-01 | 6.455e-01 | 1.000e+00 |
A_-5 | *, T | 2 | 8.755e-01 | 6.455e-01 | 1.000e+00 |
A_284 | *, I | 2 | 8.755e-01 | 6.455e-01 | 1.000e+00 |
C_156 | W, R, L, Q | 4 | 2.473e+00 | 6.495e-01 | 1.000e+00 |
A_66 | N, K | 2 | 8.571e-01 | 6.515e-01 | 1.000e+00 |
A_142 | I, T | 2 | 8.559e-01 | 6.519e-01 | 1.000e+00 |
A_145 | R, H | 2 | 8.559e-01 | 6.519e-01 | 1.000e+00 |
A_-15 | * | 1 | 2.024e-01 | 6.528e-01 | 1.000e+00 |
A_294 | * | 1 | 2.024e-01 | 6.528e-01 | 1.000e+00 |
A_79 | R, G | 2 | 8.418e-01 | 6.565e-01 | 1.000e+00 |
A_80 | I, T | 2 | 8.418e-01 | 6.565e-01 | 1.000e+00 |
A_81 | A, L | 2 | 8.418e-01 | 6.565e-01 | 1.000e+00 |
A_82 | L, R | 2 | 8.418e-01 | 6.565e-01 | 1.000e+00 |
A_83 | R, G | 2 | 8.418e-01 | 6.565e-01 | 1.000e+00 |
DPB1_96 | *, K, R | 3 | 1.606e+00 | 6.581e-01 | 1.000e+00 |
DPB1_170 | *, I, T | 3 | 1.606e+00 | 6.581e-01 | 1.000e+00 |
A_99 | Y, F | 2 | 8.308e-01 | 6.601e-01 | 1.000e+00 |
B_177 | D, E | 2 | 8.213e-01 | 6.632e-01 | 1.000e+00 |
B_180 | E, Q | 2 | 8.213e-01 | 6.632e-01 | 1.000e+00 |
B_24 | S, T, A | 3 | 1.536e+00 | 6.740e-01 | 1.000e+00 |
DRB1_31 | F, I | 2 | 7.443e-01 | 6.893e-01 | 1.000e+00 |
B_156 | R, L, W, D | 4 | 2.250e+00 | 6.899e-01 | 1.000e+00 |
A_-11 | *, S | 2 | 7.305e-01 | 6.940e-01 | 1.000e+00 |
A_65 | R, G | 2 | 6.831e-01 | 7.107e-01 | 1.000e+00 |
A_-21 | *, M | 2 | 6.789e-01 | 7.122e-01 | 1.000e+00 |
DPB1_57 | E, D | 2 | 6.767e-01 | 7.129e-01 | 1.000e+00 |
A_95 | I, V, L | 3 | 1.360e+00 | 7.148e-01 | 1.000e+00 |
A_127 | N, K | 2 | 6.643e-01 | 7.174e-01 | 1.000e+00 |
C_177 | E, K | 2 | 6.392e-01 | 7.264e-01 | 1.000e+00 |
C_97 | W, R | 2 | 6.277e-01 | 7.306e-01 | 1.000e+00 |
A_44 | R, K | 2 | 6.239e-01 | 7.320e-01 | 1.000e+00 |
A_67 | V, M | 2 | 6.239e-01 | 7.320e-01 | 1.000e+00 |
A_150 | A, V | 2 | 6.239e-01 | 7.320e-01 | 1.000e+00 |
A_158 | A, V | 2 | 6.239e-01 | 7.320e-01 | 1.000e+00 |
A_276 | *, P | 2 | 6.091e-01 | 7.374e-01 | 1.000e+00 |
A_321 | *, S | 2 | 6.091e-01 | 7.374e-01 | 1.000e+00 |
DPB1_205 | *, V, M | 3 | 1.192e+00 | 7.550e-01 | 1.000e+00 |
A_298 | *, I | 2 | 5.482e-01 | 7.603e-01 | 1.000e+00 |
A_307 | *, M | 2 | 5.482e-01 | 7.603e-01 | 1.000e+00 |
DRB1_85 | V, A | 2 | 4.685e-01 | 7.912e-01 | 1.000e+00 |
A_246 | *, A | 2 | 4.583e-01 | 7.952e-01 | 1.000e+00 |
A_70 | H, Q | 2 | 4.549e-01 | 7.965e-01 | 1.000e+00 |
DQA1_25 | Y, F | 2 | 4.525e-01 | 7.975e-01 | 1.000e+00 |
B_95 | L, W, I | 3 | 9.848e-01 | 8.049e-01 | 1.000e+00 |
A_116 | D, Y, H | 3 | 8.461e-01 | 8.384e-01 | 1.000e+00 |
DPB1_65 | I, L | 2 | 3.524e-01 | 8.385e-01 | 1.000e+00 |
DPB1_35 | F, L, Y | 3 | 8.446e-01 | 8.388e-01 | 1.000e+00 |
C_6 | R, K | 2 | 3.499e-01 | 8.395e-01 | 1.000e+00 |
A_17 | R, S | 2 | 3.409e-01 | 8.433e-01 | 1.000e+00 |
A_62 | R, G, E, Q, L | 5 | 2.026e+00 | 8.456e-01 | 1.000e+00 |
C_9 | Y, D, F, S | 4 | 1.236e+00 | 8.722e-01 | 1.000e+00 |
A_299 | *, T | 2 | 2.288e-01 | 8.919e-01 | 1.000e+00 |
A_334 | *, V | 2 | 2.288e-01 | 8.919e-01 | 1.000e+00 |
DQB1_26 | L, Y, G | 3 | 6.006e-01 | 8.963e-01 | 1.000e+00 |
DQA1_34 | Q, E | 2 | 1.851e-01 | 9.116e-01 | 1.000e+00 |
A_152 | E, V, A, R | 4 | 9.529e-01 | 9.169e-01 | 1.000e+00 |
B_74 | D, Y | 2 | 1.542e-01 | 9.258e-01 | 1.000e+00 |
C_116 | S, F, Y, L | 4 | 7.424e-01 | 9.460e-01 | 1.000e+00 |
C_16 | G, S | 2 | 1.028e-01 | 9.499e-01 | 1.000e+00 |
A_97 | R, M, I | 3 | 2.072e-01 | 9.764e-01 | 1.000e+00 |
A_166 | E, D | 2 | 2.644e-02 | 9.869e-01 | 1.000e+00 |
A_167 | W, G | 2 | 2.644e-02 | 9.869e-01 | 1.000e+00 |
A_114 | Q, H, R, E | 4 | 2.952e-01 | 9.901e-01 | 1.000e+00 |
DPA1_91 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DPA1_111 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DPA1_127 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DPA1_160 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DPA1_190 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DPA1_228 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-27 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-21 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-9 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-6 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-5 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_-4 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_203 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_220 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_221 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DQB1_224 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DRB1_-16 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DRB1_38 | V, L | 2 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DRB1_189 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DRB1_207 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
DRB1_233 | * | 0 | 0.000e+00 | 1.000e+00 | 1.000e+00 |
Next, we can investigate how effect estimates are distributed for a given associated amino acid position, e.g. DQB1_9:
HLA_AA_DQB1_9_results <- runMiDAS(
HLA_AA_model,
experiment = "hla_aa",
inheritance_model = "dominant",
omnibus_groups_filter = "DQB1_9",
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
correction = "bonferroni",
exponentiate = TRUE
)
kableResults(HLA_AA_DQB1_9_results, scroll_box_height = "250px")
aa | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | Ntotal | Ntotal [%] | N(disease=0) | N(disease=0) [%] | N(disease=1) | N(disease=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DQB1_9_F | 7.000e-07 | 2.000e-06 | 2.0572 | 0.1451 | 1.5509 | 2.740 | 4.973 | 277 | 13.85% | 103 | 10.30% | 174 | 17.40% |
DQB1_9_Y | 1.103e-01 | 3.308e-01 | 0.5876 | 0.3329 | 0.2994 | 1.116 | -1.597 | 960 | 48.00% | 485 | 48.50% | 475 | 47.50% |
DQB1_9_* | 1.000e+00 | 1.000e+00 | 1.0000 | 0.2167 | 0.6531 | 1.531 | 0.000 | 94 | 4.70% | 47 | 4.70% | 47 | 4.70% |
This shows us that individuals carrying a Phenylalanine (F) at position 9 of DQB1 have a significantly increased risk, whereas individuals carrying a Tyrosine (Y) at the same amino acid position have a decreased risk.
It is logical to hypothesize that the risk residue is found on HLA-DQB1*06:02, the previously associated HLA risk allele. MiDAS thus provides the function getAllelesforAA
to map amino acid residues back to the respective HLA alleles.
HLA-DQB1 (9) | HLA-DQB1 alleles | count | frequency |
---|---|---|---|
|
05:03, 06:01 | 97 | 4.85% |
F | 04:01, 04:02, 04:23, 06:02 | 302 | 15.10% |
Y | 02:01, 02:02, 02:10, 03:01, 03:02, 03:03, 03:04, 03:05, 03:19, 03:22, 03:251, 03:96, 05:01, 05:02, 05:04, 05:107, 06:03, 06:04, 06:07, 06:09 | 1601 | 80.05% |
Finally, it is also interesting to note that there are several amino acid positions coming up that determine the Bw4 binding motif (e.g. B_81), which is a determinant for interactions of HLA class I alleles with KIR on Natural Killer cells.
Now that we have performed KIR genotyping, or e.g. inferred KIR types from available whole-genome sequencing data, we can import this information, and check the gene frequencies. In our example, we could successfully infer KIR gene presence status for 935 out of the 1,000 individuals in our data set.
ID | KIR3DL3 | KIR2DS2 | KIR2DL2 | KIR2DL3 | KIR2DP1 | KIR2DL1 | KIR3DP1 | KIR2DL4 | KIR3DL1 | KIR3DS1 | KIR2DL5 | KIR2DS3 | KIR2DS5 | KIR2DS4 | KIR2DS1 | KIR3DL2 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
C001 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C002 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C003 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C004 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C005 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C006 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C007 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C008 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C009 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C010 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C011 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C012 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C013 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C014 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C015 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C016 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C017 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C018 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C019 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C020 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C021 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C022 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C023 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C024 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C025 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C026 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C027 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C028 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C029 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C030 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C031 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C032 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C033 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C034 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C035 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C036 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C037 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C038 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C039 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 |
C040 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C041 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C042 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C043 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C044 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C045 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C046 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C047 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C048 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C049 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C050 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C051 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C052 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C053 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C054 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C055 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C056 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C057 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C058 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C059 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C060 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C061 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C062 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C063 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C064 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C065 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C066 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C067 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C068 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C069 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C070 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C071 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C072 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C073 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C074 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C075 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C076 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C077 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C078 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C079 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C080 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C081 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C082 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C083 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C084 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C085 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C086 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C087 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C088 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C089 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C090 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C091 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C092 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C093 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C094 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C095 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C096 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C097 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C098 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C099 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C100 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C101 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C102 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C103 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C104 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C105 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C106 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C107 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C108 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C109 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C110 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C111 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C112 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C113 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C114 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C115 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C116 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C117 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C118 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C119 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C120 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C121 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C122 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C123 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C124 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C125 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C126 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C127 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C128 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C129 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C130 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C131 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C132 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C133 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C134 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C135 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C136 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C137 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C138 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C139 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C140 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C141 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C142 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C143 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C144 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C145 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C146 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C147 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C148 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C149 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C150 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C151 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C152 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C153 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C154 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C155 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C156 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C157 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C158 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C159 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C160 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C161 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C162 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C163 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C164 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C165 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C166 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C167 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C168 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C169 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C170 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C171 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C172 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C173 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C174 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C175 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C176 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C177 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C178 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C179 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C180 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C181 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C182 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C183 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C184 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C185 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C186 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C187 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C188 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C189 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C190 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C191 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C192 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C193 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C194 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C195 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C196 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C197 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C198 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C199 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C200 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C201 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
C202 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C203 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C204 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C205 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C206 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
C207 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C208 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C209 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C210 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C211 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
C212 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C213 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
C214 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C215 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C216 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C217 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C218 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C219 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C220 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C221 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C222 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C223 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C224 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C225 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C226 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C227 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C228 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C229 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C230 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C231 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C232 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C233 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C234 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C235 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C236 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C237 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C238 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C239 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C240 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C241 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C242 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C243 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C244 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C245 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C246 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C247 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C248 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C249 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C250 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C251 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C252 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C253 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C254 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C255 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C256 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C257 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C258 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C259 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C260 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C261 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C262 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C263 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C264 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C265 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C266 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C267 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C268 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C269 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C270 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C271 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C272 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C273 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C274 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C275 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C276 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C277 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C278 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C279 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C280 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C281 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C282 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C283 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C284 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C285 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C286 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C287 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C288 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C289 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C290 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C291 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C292 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C293 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C294 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C295 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C296 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C297 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C298 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C299 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C300 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C301 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C302 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C303 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C304 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C305 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C306 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C307 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C308 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C309 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C310 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C311 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C312 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C313 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C314 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C315 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C316 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C317 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C318 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C319 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C320 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C321 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C322 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C323 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C324 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C325 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C326 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C327 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
C328 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C329 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C330 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C331 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C332 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C333 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C334 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C335 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C336 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C337 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C338 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C339 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C340 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C341 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C342 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C343 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C344 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C345 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C346 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C347 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C348 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C349 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C350 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C351 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C352 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C353 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C354 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C355 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C356 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C357 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C358 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C359 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C360 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C361 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C362 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C363 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C364 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C365 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C366 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C367 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
C368 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C369 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C370 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C371 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C372 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C373 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C374 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C375 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C376 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C377 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C378 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C379 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C380 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C381 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C382 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C383 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C384 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C385 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C386 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C387 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C388 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C389 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C390 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C391 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C392 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C393 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C394 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C395 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C396 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C397 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C398 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
C399 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C400 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C401 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C402 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C403 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C404 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C405 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C406 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C407 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C408 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C409 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C410 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C411 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
C412 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C413 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C414 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C415 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C416 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C417 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C418 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C419 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C420 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C421 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C422 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C423 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C424 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C425 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C426 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C427 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C428 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C429 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C430 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C431 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C432 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C433 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C434 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C435 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C436 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C437 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C438 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C439 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C440 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C441 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C442 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C443 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C444 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C445 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C446 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C447 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C448 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C449 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C450 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
C451 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C452 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C453 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C454 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
C455 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C456 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C457 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
C458 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C459 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C460 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C461 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C462 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C463 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C464 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C465 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C466 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C467 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C468 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C469 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C470 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C471 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C472 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C473 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C474 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C475 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C476 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C477 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C478 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C479 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C480 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
C481 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C482 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C483 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C484 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C485 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C486 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C487 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
C488 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C489 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C490 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C491 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
C492 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C493 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C494 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
C495 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C496 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C497 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
C498 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C499 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
C500 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P001 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P002 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P003 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P004 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P005 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P006 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P007 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P008 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P009 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P010 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P011 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P012 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P013 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P014 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P015 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P016 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P017 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P018 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P019 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P020 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P021 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P022 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P023 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 |
P024 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P025 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P026 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P027 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P028 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P029 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P030 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P031 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
P032 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P033 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P034 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P035 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P036 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P037 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P038 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P039 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P040 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P041 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P042 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P043 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P044 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P045 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P046 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P047 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P048 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P049 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P050 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P051 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P052 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P053 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P054 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P055 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P056 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P057 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P058 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P059 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P060 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P061 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P062 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P063 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P064 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P065 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P066 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P067 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P068 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P069 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P070 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P071 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P072 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P073 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P074 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P075 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P076 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P077 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P078 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P079 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P080 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P081 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P082 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P083 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P084 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P085 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P086 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P087 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P088 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P089 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P090 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P091 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P092 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P093 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P094 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P095 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P096 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P097 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P098 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P099 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P100 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P101 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P102 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P103 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P104 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P105 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P106 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P107 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P108 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P109 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P110 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P111 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P112 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
P113 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P114 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P115 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P116 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P117 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P118 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P119 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P120 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P121 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P122 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P123 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P124 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P125 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P126 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P127 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P128 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P129 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P130 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P131 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P132 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P133 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P134 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P135 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P136 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P137 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P138 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P139 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P140 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P141 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P142 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P143 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P144 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P145 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P146 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P147 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P148 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P149 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P150 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P151 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P152 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P153 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P154 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P155 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P156 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P157 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P158 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P159 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P160 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P161 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P162 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P163 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P164 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P165 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P166 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P167 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P168 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P169 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P170 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P171 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P172 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P173 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P174 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P175 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P176 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P177 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P178 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P179 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P180 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P181 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P182 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P183 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P184 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P185 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P186 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P187 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P188 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P189 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P190 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P191 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P192 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P193 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P194 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P195 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P196 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P197 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P198 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P199 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P200 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P201 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P202 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P203 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P204 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P205 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P206 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P207 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P208 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P209 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P210 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P211 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P212 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P213 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P214 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P215 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P216 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P217 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P218 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P219 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P220 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P221 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P222 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P223 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P224 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P225 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P226 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P227 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
P228 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P229 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P230 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P231 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P232 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P233 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P234 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P235 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P236 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P237 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P238 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P239 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P240 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P241 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P242 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P243 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P244 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P245 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P246 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P247 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P248 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P249 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P250 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P251 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P252 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P253 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P254 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P255 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P256 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P257 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P258 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P259 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P260 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P261 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P262 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P263 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P264 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P265 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P266 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P267 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P268 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P269 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P270 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P271 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P272 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P273 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P274 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P275 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P276 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P277 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P278 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P279 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P280 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P281 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P282 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P283 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P284 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P285 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
P286 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P287 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P288 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P289 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P290 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P291 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P292 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P293 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P294 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P295 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P296 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P297 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P298 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P299 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P300 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P301 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P302 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P303 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P304 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P305 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P306 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P307 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P308 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P309 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P310 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P311 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P312 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P313 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P314 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P315 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P316 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P317 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P318 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P319 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P320 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P321 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P322 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P323 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P324 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P325 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P326 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P327 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P328 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P329 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P330 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P331 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P332 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P333 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P334 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P335 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P336 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P337 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P338 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P339 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P340 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P341 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P342 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P343 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P344 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P345 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P346 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P347 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P348 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P349 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P350 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P351 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P352 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P353 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P354 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P355 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P356 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P357 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P358 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P359 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P360 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P361 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P362 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P363 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P364 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P365 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P366 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P367 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P368 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P369 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P370 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P371 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P372 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P373 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P374 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P375 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P376 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P377 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P378 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P379 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P380 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P381 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P382 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P383 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P384 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P385 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P386 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P387 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P388 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P389 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P390 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
P391 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P392 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P393 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P394 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P395 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P396 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P397 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P398 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P399 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P400 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P401 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P402 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P403 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P404 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P405 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P406 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P407 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P408 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P409 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P410 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P411 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P412 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P413 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P414 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P415 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P416 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P417 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P418 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P419 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P420 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P421 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P422 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P423 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P424 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P425 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P426 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P427 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P428 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P429 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P430 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P431 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P432 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P433 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P434 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P435 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P436 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P437 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P438 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
P439 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P440 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P441 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P442 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 |
P443 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P444 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P445 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P446 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P447 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P448 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P449 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P450 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P451 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P452 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P453 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P454 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P455 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P456 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P457 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 |
P458 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P459 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P460 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P461 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P462 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P463 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P464 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P465 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P466 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P467 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P468 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P469 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P470 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P471 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P472 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P473 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P474 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P475 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P476 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P477 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P478 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P479 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P480 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P481 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P482 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
P483 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P484 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
P485 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P486 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P487 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P488 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
P489 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P490 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P491 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P492 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P493 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
P494 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P495 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P496 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P497 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
P498 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
P499 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
P500 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Next, we want to check our KIR genes frequencies.
gene | Counts | Freq |
---|---|---|
KIR3DL3 | 935 | 1.0000000 |
KIR2DS2 | 455 | 0.4866310 |
KIR2DL2 | 449 | 0.4802139 |
KIR2DL3 | 849 | 0.9080214 |
KIR2DP1 | 925 | 0.9893048 |
KIR2DL1 | 918 | 0.9818182 |
KIR3DP1 | 935 | 1.0000000 |
KIR2DL4 | 935 | 1.0000000 |
KIR3DL1 | 853 | 0.9122995 |
KIR3DS1 | 365 | 0.3903743 |
KIR2DL5 | 485 | 0.5187166 |
KIR2DS3 | 294 | 0.3144385 |
KIR2DS5 | 288 | 0.3080214 |
KIR2DS4 | 853 | 0.9122995 |
KIR2DS1 | 371 | 0.3967914 |
KIR3DL2 | 935 | 1.0000000 |
Next, we rerun our prepareMiDAS
function, this time including the KIR data. We prepare the data to test for both KIR gene associations, as well as HLA-KIR interactions. But first, let’s runMiDAS
on the level of KIR genes.
HLAKIR <- prepareMiDAS(
hla_calls = dat_HLA,
kir_calls = dat_KIR,
colData = dat_pheno,
experiment = c("hla_NK_ligands","kir_genes", "hla_kir_interactions")
)
KIR_model <- glm(disease ~ term, data = HLAKIR, family = binomial())
KIR_results <- runMiDAS(
KIR_model,
experiment = "kir_genes",
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
exponentiate = TRUE
)
kableResults(KIR_results)
kir.gene | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | Ntotal | Ntotal [%] | N(disease=0) | N(disease=0) [%] | N(disease=1) | N(disease=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KIR3DL1 | 3.700e-06 | 3.650e-05 | 3.4291 | 0.2661 | 2.0717 | 5.9126 | 4.6303 | 853 | 91.23% | 405 | 86.72% | 448 | 95.73% |
KIR2DS4 | 3.700e-06 | 3.650e-05 | 3.4291 | 0.2661 | 2.0717 | 5.9126 | 4.6303 | 853 | 91.23% | 405 | 86.72% | 448 | 95.73% |
KIR2DL3 | 4.414e-02 | 4.414e-01 | 0.6282 | 0.2310 | 0.3965 | 0.9835 | -2.0128 | 849 | 90.80% | 433 | 92.72% | 416 | 88.89% |
KIR2DS2 | 8.295e-02 | 8.295e-01 | 1.2552 | 0.1311 | 0.9710 | 1.6235 | 1.7338 | 455 | 48.66% | 214 | 45.82% | 241 | 51.50% |
KIR2DL2 | 1.087e-01 | 1.000e+00 | 1.2341 | 0.1311 | 0.9546 | 1.5962 | 1.6043 | 449 | 48.02% | 212 | 45.40% | 237 | 50.64% |
KIR2DS5 | 1.143e-01 | 1.000e+00 | 0.7992 | 0.1420 | 0.6046 | 1.0552 | -1.5790 | 288 | 30.80% | 155 | 33.19% | 133 | 28.42% |
KIR2DS3 | 1.658e-01 | 1.000e+00 | 1.2160 | 0.1411 | 0.9224 | 1.6044 | 1.3858 | 294 | 31.44% | 137 | 29.34% | 157 | 33.55% |
KIR2DS1 | 1.949e-01 | 1.000e+00 | 0.8407 | 0.1338 | 0.6465 | 1.0927 | -1.2962 | 371 | 39.68% | 195 | 41.76% | 176 | 37.61% |
KIR3DS1 | 7.178e-01 | 1.000e+00 | 0.9527 | 0.1341 | 0.7324 | 1.2391 | -0.3614 | 365 | 39.04% | 185 | 39.61% | 180 | 38.46% |
KIR2DL5 | 9.208e-01 | 1.000e+00 | 0.9871 | 0.1309 | 0.7636 | 1.2759 | -0.0994 | 485 | 51.87% | 243 | 52.03% | 242 | 51.71% |
We found an association of KIR3DL1 gene presence with disease status. Since HLA alleles encoding Bw4 epitopes are ligands for KIR3DL1, we can now ask the question whether patients coding for both receptor and ligand are at increased risk for our disease phenotype.
If both HLA alleles and KIR gene presence / absence information is available, MiDAS can encode experimentally validated receptor-ligand interactions, defined according to Pende et al. Please note that there is extensive allelic variation in KIR genes, as well as evidence that their interaction with HLA does not only depend on the presence of the KIR gene, but also allele status. Currently, MiDAS does not consider allelic variation in KIR, but a custom dictionary can be provided by the user (see documentation).
First let’s explore HLA-KIR interactions frequencies
term | Counts | Freq |
---|---|---|
C2_KIR2DL1 | 580 | 0.6079665 |
C1_KIR2DL2 | 373 | 0.3922187 |
B*46:01_KIR2DL2 | 4 | 0.0040000 |
B*73:01_KIR2DL2 | 0 | 0.0000000 |
C2_KIR2DL2 | 283 | 0.2966457 |
C1_KIR2DL3 | 704 | 0.7402734 |
B*46:01_KIR2DL3 | 10 | 0.0100000 |
B*73:01_KIR2DL3 | 2 | 0.0020000 |
C2_KIR2DL3 | 537 | 0.5628931 |
A*23_KIR3DL1 | 36 | 0.0363269 |
A*24_KIR3DL1 | 196 | 0.1983806 |
A*32_KIR3DL1 | 54 | 0.0541625 |
A*03_KIR3DL2 | 189 | 0.1926606 |
A*11_KIR3DL2 | 116 | 0.1161161 |
Bw4_KIR3DL1 | 680 | 0.7188161 |
Bw4(HLA-B_only)_KIR3DL1 | 585 | 0.6138510 |
C2_KIR2DS1 | 237 | 0.2484277 |
C1_KIR2DS2 | 379 | 0.3985279 |
A*11:01_KIR2DS2 | 48 | 0.0480480 |
C*02:02_KIR2DS4 | 76 | 0.0763052 |
C*04:01_KIR2DS4 | 180 | 0.1832994 |
C*05:01_KIR2DS4 | 122 | 0.1238579 |
C*01:02_KIR2DS4 | 85 | 0.0855131 |
C*14:02_KIR2DS4 | 28 | 0.0280280 |
C*16:01_KIR2DS4 | 42 | 0.0421264 |
A*11_KIR2DS4 | 102 | 0.1021021 |
C2_KIR2DS5 | 178 | 0.1865828 |
B*51_KIR3DS1 | 53 | 0.0532663 |
A03_A11_KIR2DS2 | 126 | 0.1285714 |
HLAKIR_model <- glm(disease ~ term, data = HLAKIR, family = binomial())
HLA_KIR_results <- runMiDAS(
HLAKIR_model,
experiment = "hla_kir_interactions",
lower_frequency_cutoff = 0.02,
upper_frequency_cutoff = 0.98,
exponentiate = TRUE
)
kableResults(HLA_KIR_results)
hla.kir.interaction | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | Ntotal | Ntotal [%] | N(disease=0) | N(disease=0) [%] | N(disease=1) | N(disease=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Bw4_KIR3DL1 | 6.000e-07 | 1.620e-05 | 2.0952 | 0.1487 | 1.5686 | 2.810 | 4.976e+00 | 680 | 71.88% | 306 | 64.56% | 374 | 79.24% |
Bw4(HLA-B_only)_KIR3DL1 | 6.240e-05 | 1.559e-03 | 1.7131 | 0.1345 | 1.3173 | 2.232 | 4.004e+00 | 585 | 61.39% | 262 | 55.04% | 323 | 67.71% |
C1_KIR2DS2 | 1.895e-03 | 4.737e-02 | 1.5127 | 0.1332 | 1.1656 | 1.966 | 3.106e+00 | 379 | 39.85% | 167 | 34.94% | 212 | 44.82% |
C1_KIR2DL2 | 2.877e-03 | 7.193e-02 | 1.4889 | 0.1336 | 1.1466 | 1.936 | 2.981e+00 | 373 | 39.22% | 165 | 34.52% | 208 | 43.97% |
B*51_KIR3DS1 | 1.801e-02 | 4.502e-01 | 2.0202 | 0.2973 | 1.1427 | 3.692 | 2.365e+00 | 53 | 5.33% | 18 | 3.61% | 35 | 7.04% |
A*24_KIR3DL1 | 1.111e-01 | 1.000e+00 | 1.2909 | 0.1602 | 0.9437 | 1.770 | 1.593e+00 | 196 | 19.84% | 88 | 17.81% | 108 | 21.86% |
C2_KIR2DS5 | 1.352e-01 | 1.000e+00 | 0.7793 | 0.1669 | 0.5610 | 1.080 | -1.494e+00 | 178 | 18.66% | 98 | 20.55% | 80 | 16.77% |
C2_KIR2DS1 | 1.549e-01 | 1.000e+00 | 0.8076 | 0.1502 | 0.6011 | 1.084 | -1.422e+00 | 237 | 24.84% | 128 | 26.83% | 109 | 22.85% |
A*11_KIR3DL2 | 1.712e-01 | 1.000e+00 | 0.7618 | 0.1988 | 0.5143 | 1.123 | -1.368e+00 | 116 | 11.61% | 65 | 13.00% | 51 | 10.22% |
A*11:01_KIR2DS2 | 2.415e-01 | 1.000e+00 | 0.7038 | 0.2999 | 0.3862 | 1.260 | -1.171e+00 | 48 | 4.80% | 28 | 5.60% | 20 | 4.01% |
A*03_KIR3DL2 | 3.149e-01 | 1.000e+00 | 1.1771 | 0.1622 | 0.8568 | 1.619 | 1.005e+00 | 189 | 19.27% | 88 | 18.00% | 101 | 20.53% |
C*02:02_KIR2DS4 | 3.498e-01 | 1.000e+00 | 1.2515 | 0.2399 | 0.7832 | 2.013 | 9.351e-01 | 76 | 7.63% | 34 | 6.84% | 42 | 8.42% |
A*03_A*11_KIR2DS2 | 3.529e-01 | 1.000e+00 | 1.1946 | 0.1914 | 0.8215 | 1.742 | 9.290e-01 | 126 | 12.86% | 58 | 11.86% | 68 | 13.85% |
A*11_KIR2DS4 | 4.096e-01 | 1.000e+00 | 0.8413 | 0.2096 | 0.5563 | 1.268 | -8.245e-01 | 102 | 10.21% | 55 | 11.00% | 47 | 9.42% |
C*05:01_KIR2DS4 | 4.465e-01 | 1.000e+00 | 1.1590 | 0.1938 | 0.7931 | 1.698 | 7.613e-01 | 122 | 12.39% | 57 | 11.59% | 65 | 13.18% |
C2_KIR2DL3 | 4.728e-01 | 1.000e+00 | 0.9105 | 0.1306 | 0.7048 | 1.176 | -7.179e-01 | 537 | 56.29% | 274 | 57.44% | 263 | 55.14% |
A*32_KIR3DL1 | 5.709e-01 | 1.000e+00 | 0.8530 | 0.2806 | 0.4890 | 1.478 | -5.668e-01 | 54 | 5.42% | 29 | 5.82% | 25 | 5.01% |
C*04:01_KIR2DS4 | 5.996e-01 | 1.000e+00 | 1.0905 | 0.1650 | 0.7891 | 1.508 | 5.250e-01 | 180 | 18.33% | 87 | 17.68% | 93 | 18.98% |
C2_KIR2DL2 | 6.198e-01 | 1.000e+00 | 1.0729 | 0.1418 | 0.8126 | 1.417 | 4.961e-01 | 283 | 29.66% | 138 | 28.93% | 145 | 30.40% |
C*01:02_KIR2DS4 | 7.047e-01 | 1.000e+00 | 1.0898 | 0.2270 | 0.6982 | 1.705 | 3.790e-01 | 85 | 8.55% | 41 | 8.22% | 44 | 8.89% |
C*14:02_KIR2DS4 | 7.057e-01 | 1.000e+00 | 0.8649 | 0.3843 | 0.4012 | 1.840 | -3.777e-01 | 28 | 2.80% | 15 | 3.00% | 13 | 2.61% |
A*23_KIR3DL1 | 7.483e-01 | 1.000e+00 | 1.1153 | 0.3401 | 0.5717 | 2.193 | 3.209e-01 | 36 | 3.63% | 17 | 3.44% | 19 | 3.82% |
C*16:01_KIR2DS4 | 7.576e-01 | 1.000e+00 | 1.1023 | 0.3157 | 0.5928 | 2.061 | 3.086e-01 | 42 | 4.21% | 20 | 4.02% | 22 | 4.41% |
C2_KIR2DL1 | 8.945e-01 | 1.000e+00 | 0.9826 | 0.1326 | 0.7575 | 1.274 | -1.326e-01 | 580 | 60.80% | 291 | 61.01% | 289 | 60.59% |
C1_KIR2DL3 | 9.824e-01 | 1.000e+00 | 0.9967 | 0.1479 | 0.7458 | 1.332 | -2.209e-02 | 704 | 74.03% | 354 | 74.06% | 350 | 74.00% |
Patients who are carriers of both HLA-Bw4 and KIR3DL1 are at a significantly increased risk for having the disease, which is consistent with the associations we saw on KIR ligand and KIR gene level.
Finally, let’s now take a look at some additional ways to analyze immunogenetics data within MiDAS, which are probably not relevant for most users.
Instead of focusing on single HLA alleles or their interactions, you might be interested in HLA diversity. For example, it has been shown that MHC heterozygosity confers a selective advantage against infections with multiple Salmonella strains. But given the degree of variability in HLA genes, heterozygosity is a rather crude measure. On the amino acid sequence level, it is possible to perform more refined analyses, by calculating the level of evolutionary divergence between pairs of HLA alleles of different genes. Pierini and Lenz have found Grantham’s distance score to be a good proxy for HLA functional divergence. It has been demonstrated that HLA-C allelic divergence was associated with HIV viral load. In addition, there is evidence for a role of HLA class I divergence in the efficacy of cancer immunotherapy.
MiDAS provides experiment types hla_het
to analyze heterozygosity of class I and II genes, as well as hla_divergence
to analyze classical class I genes using Grantham’s distance:
HLA_het <- prepareMiDAS(
hla_calls = dat_HLA,
colData = dat_pheno,
experiment = c("hla_het","hla_divergence")
)
HLA_het_model <- glm(outcome ~ term, data=HLA_het, family=binomial())
HLA_het_results <- runMiDAS(HLA_het_model,
experiment = "hla_het",
exponentiate = TRUE
)
kableResults(HLA_het_results)
term | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic | Ntotal | Ntotal [%] | N(outcome=0) | N(outcome=0) [%] | N(outcome=1) | N(outcome=1) [%] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
C_het | 5.405e-02 | 0.4865 | 0.5436 | 0.3165 | 0.2878 | 1.003 | -1.9264 | 900 | 90.00% | 246 | 93.18% | 208 | 88.14% |
DPA1_het | 2.155e-01 | 1.0000 | 1.2585 | 0.1856 | 0.8748 | 1.812 | 1.2386 | 350 | 35.00% | 91 | 34.47% | 94 | 39.83% |
DQA1_het | 4.297e-01 | 1.0000 | 1.2477 | 0.2802 | 0.7230 | 2.179 | 0.7897 | 868 | 86.80% | 230 | 87.12% | 211 | 89.41% |
B_het | 4.672e-01 | 1.0000 | 1.2998 | 0.3606 | 0.6455 | 2.686 | 0.7271 | 934 | 93.40% | 244 | 92.42% | 222 | 94.07% |
A_het | 5.067e-01 | 1.0000 | 1.2057 | 0.2817 | 0.6962 | 2.111 | 0.6640 | 892 | 89.20% | 231 | 87.50% | 211 | 89.41% |
DPB1_het | 5.279e-01 | 1.0000 | 1.1468 | 0.2170 | 0.7504 | 1.759 | 0.6312 | 779 | 77.90% | 203 | 76.89% | 187 | 79.24% |
DRB1_het | 6.693e-01 | 1.0000 | 0.8824 | 0.2931 | 0.4954 | 1.572 | -0.4271 | 890 | 89.00% | 238 | 90.15% | 210 | 88.98% |
DRA_het | 6.997e-01 | 1.0000 | 0.9329 | 0.1800 | 0.6553 | 1.327 | -0.3857 | 435 | 43.50% | 122 | 46.21% | 105 | 44.49% |
DQB1_het | 7.659e-01 | 1.0000 | 0.9184 | 0.2860 | 0.5234 | 1.614 | -0.2977 | 882 | 88.20% | 236 | 89.39% | 209 | 88.56% |
HLA_div_results <- runMiDAS(HLA_het_model,
experiment = "hla_divergence",
exponentiate = TRUE
)
kableResults(HLA_div_results, scroll_box_height = "250px")
term | p.value | p.adjusted | estimate | std.error | conf.low | conf.high | statistic |
---|---|---|---|---|---|---|---|
ABC_avg | 0.3713 | 1 | 1.043 | 4.668e-02 | 0.9517 | 1.143 | 0.8940 |
A | 0.4519 | 1 | 1.018 | 2.416e-02 | 0.9713 | 1.068 | 0.7522 |
B | 0.6163 | 1 | 1.013 | 2.577e-02 | 0.9632 | 1.066 | 0.5012 |
C | 0.7747 | 1 | 1.012 | 4.052e-02 | 0.9345 | 1.096 | 0.2863 |
In our example dataset, our results show that there is no significant association of HLA heterozygosity or evolutionary divergence with our disease phenotype of interest.
We hope that this tutorial is useful to get you started working with MiDAS. Please don’t hesitate to contact us if you have questions or suggestions for improvement.