HSMMSingleCell

DOI: 10.18129/B9.bioc.HSMMSingleCell    

Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM)

Bioconductor version: Release (3.15)

Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.

Author: Cole Trapnell

Maintainer: Cole Trapnell <cole at broadinstitute.org>

Installation

To install this package, start R (version "4.2") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("HSMMSingleCell")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

PDF		Reference Manual

Details

biocViews	ExperimentData, RNASeqData
Version	1.16.0
License	Artistic-2.0
Depends	R (>= 2.10)
Imports
LinkingTo
Suggests
SystemRequirements
Enhances
URL
Depends On Me
Imports Me	monocle
Suggests Me	cellTree, ctgGEM
Links To Me
Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package	HSMMSingleCell_1.16.0.tar.gz
Windows Binary
macOS Binary (x86_64)
Source Repository	git clone https://git.bioconductor.org/packages/HSMMSingleCell
Source Repository (Developer Access)	git clone git@git.bioconductor.org:packages/HSMMSingleCell
Package Short Url	https://bioconductor.org/packages/HSMMSingleCell/
Package Downloads Report	Download Stats