RNAdecay

DOI: 10.18129/B9.bioc.RNAdecay    

Maximum Likelihood Decay Modeling of RNA Degradation Data

Bioconductor version: Release (3.14)

RNA degradation is monitored through measurement of RNA abundance after inhibiting RNA synthesis. This package has functions and example scripts to facilitate (1) data normalization, (2) data modeling using constant decay rate or time-dependent decay rate models, (3) the evaluation of treatment or genotype effects, and (4) plotting of the data and models. Data Normalization: functions and scripts make easy the normalization to the initial (T0) RNA abundance, as well as a method to correct for artificial inflation of Reads per Million (RPM) abundance in global assessments as the total size of the RNA pool decreases. Modeling: Normalized data is then modeled using maximum likelihood to fit parameters. For making treatment or genotype comparisons (up to four), the modeling step models all possible treatment effects on each gene by repeating the modeling with constraints on the model parameters (i.e., the decay rate of treatments A and B are modeled once with them being equal and again allowing them to both vary independently). Model Selection: The AICc value is calculated for each model, and the model with the lowest AICc is chosen. Modeling results of selected models are then compiled into a single data frame. Graphical Plotting: functions are provided to easily visualize decay data model, or half-life distributions using ggplot2 package functions.

Author: Reed Sorenson [aut, cre], Katrina Johnson [aut], Frederick Adler [aut], Leslie Sieburth [aut]

Maintainer: Reed Sorenson <reedssorenson at gmail.com>

Installation

To install this package, start R (version "4.1") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("RNAdecay")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("RNAdecay")

 

HTML	R Script	RNAdecay
PDF		Reference Manual
Text		NEWS

Details

biocViews	DifferentialExpression, GeneExpression, GeneRegulation, ImmunoOncology, Normalization, RNASeq, Regression, Software, TimeCourse, Transcription, Transcriptomics, WorkflowStep
Version	1.14.0
In Bioconductor since	BioC 3.7 (R-3.5) (4 years)
License	GPL-2
Depends	R (>= 3.5)
Imports	stats, grDevices, grid, ggplot2, gplots, utils, TMB, nloptr, scales
LinkingTo
Suggests	parallel, knitr, reshape2, rmarkdown
SystemRequirements
Enhances
URL
Depends On Me
Imports Me
Suggests Me
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Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package	RNAdecay_1.14.0.tar.gz
Windows Binary
macOS 10.13 (High Sierra)	RNAdecay_1.14.0.tgz
Source Repository	git clone https://git.bioconductor.org/packages/RNAdecay
Source Repository (Developer Access)	git clone git@git.bioconductor.org:packages/RNAdecay
Package Short Url	https://bioconductor.org/packages/RNAdecay/
Package Downloads Report	Download Stats
Old Source Packages for BioC 3.14	Source Archive