pre_processRDS {scMAGeCK} | R Documentation |
Pre-process the sgRNA count from previous step, and generate the sgRNA expression matrix.
pre_processRDS(BARCODE, RDS)
BARCODE |
A txt file to include cell identity information, generated from the cell identity collection step. |
RDS |
A Seurat object or local RDS file path that contains the scRNA-seq dataset. Note that the dataset has to be normalized and scaled. |
### BARCODE file contains cell identity information, generated from the cell identity collection step BARCODE <- system.file("extdata","barcode_rec.txt",package = "scMAGeCK") ### RDS can be a Seurat object or local RDS file path that contains the scRNA-seq dataset RDS <- system.file("extdata","singles_dox_mki67_v3.RDS",package = "scMAGeCK") Demo <- pre_processRDS(BARCODE = BARCODE, RDS = RDS)