calibrate_per_cell_total_proposal {monocle} | R Documentation |
Calibrate_per_cell_total_proposal
calibrate_per_cell_total_proposal( relative_exprs_matrix, t_estimate, expected_capture_rate, method = c("num_genes", "tpm_fraction") )
relative_exprs_matrix |
The matrix of relative TPM expression values |
t_estimate |
the TPM value that corresponds to 1 cDNA copy per cell |
expected_capture_rate |
The fraction of mRNAs captured as cDNAs |
method |
the formula to estimate the total mRNAs (num_genes corresponds to the second formula while tpm_fraction corresponds to the first formula, see the anouncement on Trapnell lab website for the Census paper) |