calibrate_per_cell_total_proposal {monocle}R Documentation

Calibrate_per_cell_total_proposal

Description

Calibrate_per_cell_total_proposal

Usage

calibrate_per_cell_total_proposal(
  relative_exprs_matrix,
  t_estimate,
  expected_capture_rate,
  method = c("num_genes", "tpm_fraction")
)

Arguments

relative_exprs_matrix

The matrix of relative TPM expression values

t_estimate

the TPM value that corresponds to 1 cDNA copy per cell

expected_capture_rate

The fraction of mRNAs captured as cDNAs

method

the formula to estimate the total mRNAs (num_genes corresponds to the second formula while tpm_fraction corresponds to the first formula, see the anouncement on Trapnell lab website for the Census paper)


[Package monocle version 2.22.0 Index]