normalizePlateEffect {RNAinteract} | R Documentation |
Normalization of plate effects in the screen.
normalizePlateEffect(sgi, type = "Bscore", maxit = 20, verbose = 0)
sgi |
An object of class |
type |
If type is "Bscore" (default) a Bscore-normalization is performed. If type is "spatial", a locfit regression is estimated that accounts for spatial effects. |
maxit |
Maximum number of iterations for locfit. |
verbose |
Either 0 (default, no output), 1 (minimum output), or 2 (outout). |
The Bscore normalization estimates row and column effects for each plate. It returns the residuals to the sum of row and column effects. The spatial normalization estimates a non-linear 2D regression for each plate and returns the residuals.
An object of class RNAinteract
. The returned object contains the normalization information.
Bernd Fischer
data("sgi") normalizePlateEffect(sgi)