Description
Read distribution plot - plot shows number of reads assigned during read grouping
Filtered Reads - plot shows percentage of assigned reads that have been recognized as PRIMER DIMERS or filtered based on low alignment score
Edit rates - plot gives overview of percentage of reads (not filtered as PRIMER DIMER) that have edits
Frameshift - plot shows what percentage of reads that have frameshift
Read heterogeneity plot - shows what is the share of each of the unique reads in total count of all reads. The more yellow each row, the less heterogeneity in the reads, more black means reads don’t repeat often and are unique
Top unassigned reads - take a look at the alignment of most abundant forward and reverse complemented reverse reads for each barcode, if you find that there is many unassigned reads you can ivestigate here.
Barcode Summary
Groups IDs
barcode_1 |
ID_1, ID_2 |
barcode_2 |
ID_3, ID_4, ID_5 |
Read distribution
Filtered Reads
Edit rates
Frameshift
Heterogeneity of reads
Top unassigned reads
barcode_1
P1 1 AAAT--ACTGTCTTGTGACCAAACCTTCTTAAGGTGCTGTTTT-GATGAT 47
|| || | ||| ||| ||| || ||||| | || |
S1 1 AAGCTGACGGCTAAATGA--AAAATATCTGAAACATCTGTTCCAGGTGCT 48
P1 48 AAACTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTTGGCAGAGGGTCA 97
| | || | || | | | | | | | |||||
S1 49 GCGTATGCCAGGGCAGA-GAAGAAG-GTCAGGGAAGGTCACTGGAGGTCA 96
P1 98 ---GCAGACCAGTAAGTCTTCTCAATTTCTTTTATTTATGTATATGTAGT 144
| | ||| | ||||| || | | | |
S1 97 CTGGGATACCCTT---TCTTCCCACACCAATGGGGAAAGGAGTCCTGCCA 143
P1 145 GATAAA-A 151
||| | |
S1 144 GATGACCA 151