intensity2circle {peco} | R Documentation |
We use FUCCI intensities to infer the position of the cells in cell cycle progression. The result is a vector of angles on a unit circle corresponding to the positions of the cells in cell cycle progression.
intensity2circle(mat, plot.it = FALSE, method = c("trig", "algebraic"))
mat |
A matrix of two columns of summarized fluorescence intensity. Rows correspond to samples. |
plot.it |
TRUE or FALSE. Plot the fitted results. |
method |
The method used to fit the circle. |
The inferred angles on unit circle based on the input intensity measurements.
Joyce Hsiao
# use our data library(SingleCellExperiment) data(sce_top101genes) # FUCCI scores - log10 transformed sum of intensities that were # corrected for background noise ints <- colData(sce_top101genes)[,c("rfp.median.log10sum.adjust", "gfp.median.log10sum.adjust")] intensity2circle(ints, plot.it=TRUE, method = "trig")