newFlowframe {cyanoFilter}R Documentation

takes a flowframe, a group indicator and formulates another flowframe with group indicator as part of the expression matrix of the new flowframe.

Description

takes a flowframe, a group indicator and formulates another flowframe with group indicator as part of the expression matrix of the new flowframe.

Usage

newFlowframe(flowfile, group = NULL, togate = NULL)

Arguments

flowfile

flowframe after debris are removed.

group

cluster group to be added to the expression matrix

togate

channel detected to have more than one peak

Value

flowframe with indicators for particle cluster

Examples

flowfile_path <- system.file("extdata", "B4_18_1.fcs", 
                  package = "cyanoFilter",
              mustWork = TRUE)
flowfile <- flowCore::read.FCS(flowfile_path, alter.names = TRUE,
                               transformation = FALSE, emptyValue = FALSE,
                               dataset = 1) 
flowfile_nona <- cyanoFilter::noNA(x = flowfile)
flowfile_noneg <- cyanoFilter::noNeg(x = flowfile_nona)
flowfile_logtrans <- cyanoFilter::lnTrans(x = flowfile_noneg, 
c('SSC.W', 'TIME'))
oneDgate(flowfile, 'RED.B.HLin')


[Package cyanoFilter version 1.0.0 Index]