oneDgate {cyanoFilter} | R Documentation |
returns the labels stating the cluster of each row in a flowfile.
oneDgate(flowfile, togate)
flowfile |
flowframe after debris are removed. |
togate |
channels detected to have more than one peak present.
Provide by the |
list of indicators for cells above and below an estimated threshold
flowfile_path <- system.file("extdata", "B4_18_1.fcs", package = "cyanoFilter", mustWork = TRUE) flowfile <- flowCore::read.FCS(flowfile_path, alter.names = TRUE, transformation = FALSE, emptyValue = FALSE, dataset = 1) flowfile_nona <- cyanoFilter::noNA(x = flowfile) flowfile_noneg <- cyanoFilter::noNeg(x = flowfile_nona) flowfile_logtrans <- cyanoFilter::lnTrans(x = flowfile_noneg, c('SSC.W', 'TIME')) oneDgate(flowfile, 'RED.B.HLin')