getUsedBarcodes {GUIDEseq} | R Documentation |
Create barcodes from the p5 and p7 index for assigning reads to each barcode
getUsedBarcodes(p5.index, p7.index, header = FALSE, reverse.p7 = TRUE, reverse.p5 = FALSE, outputFile)
p5.index |
A text file with one p5 index sequence per line |
p7.index |
A text file with one p7 index sequence per line |
header |
Indicate whether there is a header in the p5.index and p7.index files. Default to FALSE |
reverse.p7 |
Indicate whether to reverse p7 index, default to TRUE for standard GUIDE-seq experiments |
reverse.p5 |
Indicate whether to reverse p5 index, default to FALSE for standard GUIDE-seq experiments |
outputFile |
Give a name to the output file where the generated barcodes are written |
DNAStringSet
Create barcode file to be used to bin the reads sequenced in a pooled lane
Lihua Julie Zhu
p7 <- system.file("extdata", "p7.index", package = "GUIDEseq") p5 <- system.file("extdata", "p5.index", package = "GUIDEseq") outputFile <- "usedBarcode" getUsedBarcodes(p5.index = p5, p7.index = p7, reverse.p7 = TRUE, reverse.p5 = FALSE, outputFile = outputFile)