qpcR2CyclesSet {ReadqPCR} | R Documentation |
Transform a dataset from package qpcR into an object of class "CyclesSet"
.
qpcR2CyclesSet(x, cyc = 1, cycleThreshold)
x |
the name of the dataset from package qpcR to be transformed. |
cyc |
the column number which contains cycle data. |
cycleThreshold |
maximum number of cycles which the data will be counted and transformed. |
Allows the user to transform a dataset from package qpcR into an object of class
"CyclesSet"
class, alongside phenotypic data.
Object of class "CyclesSet"
.
Nor Izayu Abdul Rahman, Matthias Kohl Matthias.Kohl@stamats.de
Perkins, JR, Dawes, JM, McMahon, SB, Bennett, DL, Orengo, C, Kohl, M (2012). ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data. BMC Genomics, 13, 1:296.
library(qpcR) ## Transform the reps dataset from qpcR Package ## with maximum cycle to be read in the value is 45. repsdata <- qpcR2CyclesSet(reps, cyc=1, cycleThreshold=45) ## Transform the batsch1 dataset from qpcR Package ## with maximum cycle to be read in the value is 40. batsch1data <- qpcR2CyclesSet(batsch1, cyc=1, cycleThreshold=40)