rmMultiPCRChimera {CrispRVariants} | R Documentation |
Finds and removes sets of chimeric read alignments that overlap more than one guide, i.e. that cannot be unambiguously assigned to a single guide.
rmMultiPCRChimera(readnames, pcrhits, chimera_idxs, ...) ## S4 method for signature 'character,Hits,integer' rmMultiPCRChimera(readnames, pcrhits, chimera_idxs, ..., verbose = TRUE)
readnames |
A set of read names, used for identifying chimeric read sets |
pcrhits |
A mapping between indices of reads and a set of pcr primers |
chimera_idxs |
location of chimeric reads within the bam |
... |
Additional arguments |
verbose |
Display information about the chimeras (Default: TRUE) |
pcrhits, with chimeric reads mapping to different primers omitted.
Helen Lindsay