spotCenter {CONFESS} | R Documentation |
It estimates a series of spot statistics on each channel.
spotCenter(img, foregroundCut, howbig, ImgLimits)
img |
Data matrix. The channel image data. |
foregroundCut |
Numeric vector. The binary segmentation image analysis cutoffs for normalized image data. Pixels with normalized signals higher than the cutoff belong to foreground. |
howbig |
Integer. An user defined value of the minimum expected spot size. |
ImgLimits |
a cutoff that determines where the spot is supposed to be found. |
A list of spot coordinate estimates