SeSAMe can be converted back and forth from Minfi RGChannelSet in multiple ways. One can sesamize a minfi RGChannelSet which returns a GenomicRatioSet. Here we are illustrating using the FlowSorted.Blood.450k object, which is distributed in the minfi::RGChannelSet.
## Sesamizing WB_105...## Sesamizing WB_218...## Sesamizing WB_261...## Sesamizing PBMC_105...## Loading required package: IlluminaHumanMethylation450kanno.ilmn12.hg19## Loading required package: IlluminaHumanMethylation450kmanifest## class: GenomicRatioSet
## dim: 485512 4
## metadata(1): SNPs
## assays(2): Beta CN
## rownames(485512): cg13869341 cg14008030 ... cg08265308 cg14273923
## rowData names(0):
## colnames(4): WB_105 WB_218 WB_261 PBMC_105
## colData names(8): Sample_Name Slide ... CellType Sex
## Annotation
## array: IlluminaHumanMethylation450k
## annotation: ilmn12.hg19
## Preprocessing
## Method: SeSAMe (type I)
## minfi version: 1.34.0
## Manifest version: 0.6.0