rice.bg {vasp} | R Documentation |
Small ballgown object created with a subset of rice RNAseq data, for demonstration purposes
rice.bg
a ballgown object with 33 transcripts and 6 samples
The raw RNA-seq data were screened and trimmed using Trimmomatic (Bolger et al., 2014) and RNA-seq mapping, transcript assembly, and quantification were conducted with HISAT, StringTie, and Ballgown by following the method described by Pertea et al. (Pertea et al., 2016). The rice.bg is a subset ballgown object with 33 transcripts and 6 samples. #> library(vasp) #> ?ballgown #> path<-system.file('extdata', package='vasp') #> rice.bg<-ballgown(samples = list.dirs(path = path,recursive = F))
The raw RNA-seq data were from the project of variation in transcriptional responses to salt stress in rice (SRA Accession: SRP106054).
Bolger, A.M., Lohse, M., and Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114-2120.
Pertea, M., Kim, D., Pertea, G.M., Leek, J.T., and Salzberg, S.L. (2016). Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown. Nat Protoc 11, 1650-1667.
data(rice.bg) rice.bg # ballgown instance with 33 transcripts and 6 samples