getManualCountsEstimates {frenchFISH} | R Documentation |
FrenchFISH function for generating volume adjusted spot counts from spots which have been manually counted (uses a Markov chain Monte Carlo method).
getManualCountsEstimates(probeCounts, radius, height)
probeCounts |
A matrix of manual spot counts with columns for probes and rows for nuclei |
radius |
The cells' nuclear radius (must be measured in same unit as
|
height |
The section height (must be measured in same unit as
|
The volume adjusted spot counts for each probe that have been generated using MCMC modelling
manualCountsEstimates<-getManualCountsEstimates(cbind(red=c(0,2,4), green=c(5,3,1), blue=c(3,0,2)), 8, 4)