positiveFactor {NanoStringDiff} | R Documentation |
Positive size factors can be used to adjust all platform associated sources of variation.
## S4 method for signature 'NanoStringSet' positiveFactor(object) ## S4 replacement method for signature 'NanoStringSet,numeric' positiveFactor(object) <- value
object |
A NanoStringSet object. |
value |
A vector of positive size factors. |
The observed counts including negative control genes and housekeeping control genes might be effect by some experimental factors like hybridization and binding efficiency. In order to get the true rate of gene expression, these variations must be normalized. Positive size factors can normalize this kind of variation.
A vector contain positive size factors
Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>
positiveControl
data(NanoStringData) ## obtain positive factors positiveFactor(NanoStringData) ## assign a vector n=ncol(exprs(NanoStringData)) positiveFactor(NanoStringData)=rep(1,n)