############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/R/R/bin/R CMD build --keep-empty-dirs --no-resave-data xcms ### ############################################################################## ############################################################################## * checking for file ‘xcms/DESCRIPTION’ ... OK * preparing ‘xcms’: * checking DESCRIPTION meta-information ... OK * cleaning src * installing the package to build vignettes * creating vignettes ... ERROR --- re-building ‘LC-MS-feature-grouping.Rmd’ using rmarkdown --- finished re-building ‘LC-MS-feature-grouping.Rmd’ --- re-building ‘xcms-direct-injection.Rmd’ using rmarkdown Quitting from lines 58-73 [load-data] (xcms-direct-injection.Rmd) Error: processing vignette 'xcms-direct-injection.Rmd' failed with diagnostics: inherits(x, "mzR") is not TRUE --- failed re-building ‘xcms-direct-injection.Rmd’ --- re-building ‘xcms-lcms-ms.Rmd’ using rmarkdown Quitting from lines 79-86 [load-dda-data] (xcms-lcms-ms.Rmd) Error: processing vignette 'xcms-lcms-ms.Rmd' failed with diagnostics: File(s) not found --- failed re-building ‘xcms-lcms-ms.Rmd’ --- re-building ‘xcms.Rmd’ using rmarkdown filterFeatures package:ProtGenerics R Documentation _F_i_l_t_e_r _f_e_a_t_u_r_e_s _D_e_s_c_r_i_p_t_i_o_n: Implementations of this generic filter function are supposed to filter _features_ in 'object' based on a filter criteria defined by parameter 'filter'. _U_s_a_g_e: filterFeatures(object, filter, ...) _A_r_g_u_m_e_n_t_s: object: The object to filter. filter: The filtering criteria on which 'object' should be filtered. ...: Optional parameters. BlankFlag package:xcms R Documentation _F_l_a_g _f_e_a_t_u_r_e_s _b_a_s_e_d _o_n _t_h_e _i_n_t_e_n_s_i_t_y _i_n _b_l_a_n_k _s_a_m_p_l_e_s _D_e_s_c_r_i_p_t_i_o_n: The `BlankFlag` class and method enable users to flag features of an `XcmsExperiment` or `SummarizedExperiment` object based on the relationship between the intensity of a feature in blanks compared to the intensity in the samples. This class and method are part of the possible dispatch of the generic function `filterFeatures`. Features *below* (`<`) the user-input threshold will be flagged by calling the `filterFeatures` function. This means that an extra column will be created in `featureDefinitions` or `rowData` called `possible_contaminants` with a logical value for each feature. _U_s_a_g_e: BlankFlag( threshold = 2, blankIndex = integer(), qcIndex = integer(), na.rm = TRUE ) ## S4 method for signature 'XcmsResult,BlankFlag' filterFeatures(object, filter, ...) ## S4 method for signature 'SummarizedExperiment,BlankFlag' filterFeatures(object, filter, assay = 1) _A_r_g_u_m_e_n_t_s: threshold: `numeric` indicates the minimum difference required between the mean abundance of a feature in samples compared to the mean abundance of the same feature in blanks for it to not be considered a possible contaminant. For example, the default threshold of 2 signifies that the mean abundance of the features in samples has to be at least twice the mean abundance in blanks for it to not be flagged as a possible contaminant. blankIndex: `integer` (or `logical`) vector corresponding to the indices of blank samples. qcIndex: `integer` (or `logical`) vector corresponding to the indices of quality control (QC) samples. na.rm: `logical` indicates whether missing values (`NA`) should be removed prior to the calculations. object: 'XcmsExperiment' or 'SummarizedExperiment'. For an 'XcmsExperiment' object, the 'featureValues(object)' will be evaluated, and for 'Summarizedesxperiment' the 'assay(object, assay)'. The object will be filtered. filter: The parameter object selecting and configuring the type of filtering. It can be one of the following classes: 'RsdFilter', 'DratioFilter', 'PercentMissingFilter' or 'BlankFlag'. ...: Optional parameters. For 'object' being an 'XcmsExperiment': parameters for the 'featureValues()' call. assay: For filtering of 'SummarizedExperiment' objects only. Indicates which assay the filtering will be based on. Note that the features for the entire object will be removed, but the computations are performed on a single assay. Default is 1, which means the first assay of the 'object' will be evaluated. _V_a_l_u_e: For `BlankFlag`: a `BlankFlag` class. `filterFeatures` returns the input object with an added column in the features metadata called `possible_contaminants` with a logical value for each feature. This is added to `featureDefinitions` for `XcmsExperiment` objects and `rowData` for `SummarizedExperiment` objects. _A_u_t_h_o_r(_s): Philippine Louail _S_e_e _A_l_s_o: Other Filter features in xcms: 'DratioFilter', 'PercentMissingFilter', 'RsdFilter' --- finished re-building ‘xcms.Rmd’ SUMMARY: processing the following files failed: ‘xcms-direct-injection.Rmd’ ‘xcms-lcms-ms.Rmd’ Error: Vignette re-building failed. Execution halted