############################################################################## ############################################################################## ### ### Running command: ### ### /Library/Frameworks/R.framework/Resources/bin/R CMD build --keep-empty-dirs --no-resave-data msPurity ### ############################################################################## ############################################################################## * checking for file ‘msPurity/DESCRIPTION’ ... OK * preparing ‘msPurity’: * checking DESCRIPTION meta-information ... OK * installing the package to build vignettes * creating vignettes ... ERROR --- re-building ‘msPurity-lcmsms-data-processing-and-spectral-matching-vignette.Rmd’ using rmarkdown Loading required package: Rcpp Loading required package: BiocParallel Loading required package: MSnbase Loading required package: BiocGenerics Attaching package: 'BiocGenerics' The following objects are masked from 'package:stats': IQR, mad, sd, var, xtabs The following objects are masked from 'package:base': Filter, Find, Map, Position, Reduce, anyDuplicated, append, as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted, lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which.max, which.min Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")', and for packages 'citation("pkgname")'. Loading required package: mzR Loading required package: S4Vectors Loading required package: stats4 Attaching package: 'S4Vectors' The following objects are masked from 'package:base': I, expand.grid, unname Loading required package: ProtGenerics Attaching package: 'ProtGenerics' The following object is masked from 'package:stats': smooth This is MSnbase version 2.20.4 Visit https://lgatto.github.io/MSnbase/ to get started. Attaching package: 'MSnbase' The following object is masked from 'package:base': trimws This is xcms version 3.16.1 Attaching package: 'xcms' The following object is masked from 'package:stats': sigma Warning in .local(object, ...) : It looks like this file is in profile mode. centWave can process only centroid mode data ! Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 16399 regions of interest ... OK: 9961 found. Warning in .local(object, ...) : It looks like this file is in profile mode. centWave can process only centroid mode data ! Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 16425 regions of interest ... OK: 9896 found. Warning in .local(object, ...) : It looks like this file is in profile mode. centWave can process only centroid mode data ! Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 71125 regions of interest ... OK: 32825 found. Warning in .local(object, ...) : It looks like this file is in profile mode. centWave can process only centroid mode data ! Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 71486 regions of interest ... OK: 32736 found. Processing 11756 mz slices ... OK Attaching package: 'magrittr' The following object is masked from 'package:msPurity': subtract Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 1996 regions of interest ... OK: 368 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 2104 regions of interest ... OK: 432 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 3234 regions of interest ... OK: 1107 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 3284 regions of interest ... OK: 1111 found. Processing 11756 mz slices ... OK only MS1 data No MS/MS spectra for file: /Library/Frameworks/R.framework/Versions/4.1/Resources/library/msPurityData/extdata/lcms/mzML/LCMS_1.mzML only MS1 data No MS/MS spectra for file: /Library/Frameworks/R.framework/Versions/4.1/Resources/library/msPurityData/extdata/lcms/mzML/LCMS_2.mzML convert2RawRT == TRUE but retention time alignment not applied to xcmsObj. Using raw retention times for features Creating a database of fragmentation spectra and LC features --- finished re-building ‘msPurity-lcmsms-data-processing-and-spectral-matching-vignette.Rmd’ --- re-building ‘msPurity-spectral-database-vignette.Rmd’ using rmarkdown --- finished re-building ‘msPurity-spectral-database-vignette.Rmd’ --- re-building ‘msPurity-vignette.Rmd’ using rmarkdown only MS1 data No MS/MS spectra for file: /Library/Frameworks/R.framework/Versions/4.1/Resources/library/msPurityData/extdata/lcms/mzML/LCMS_1.mzML Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 1996 regions of interest ... OK: 368 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 2104 regions of interest ... OK: 432 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 3234 regions of interest ... OK: 1107 found. Detecting mass traces at 10 ppm ... OK Detecting chromatographic peaks in 3284 regions of interest ... OK: 1111 found. Processing 11756 mz slices ... OK convert2RawRT == TRUE but retention time alignment not applied to xcmsObj. Using raw retention times for features Sizes of mz and intensity arrays don't match. Quitting from lines 175-176 (msPurity-vignette.Rmd) Error: processing vignette 'msPurity-vignette.Rmd' failed with diagnostics: dims [product 4516] do not match the length of object [2258] --- failed re-building ‘msPurity-vignette.Rmd’ SUMMARY: processing the following file failed: ‘msPurity-vignette.Rmd’ Error: Vignette re-building failed. Execution halted